Evidence map›Paper›PMID 41459214›Full record

ArticleFrontiers in microbiology2025

Integrated transcriptomic and metabolomic analyses reveal the antibacterial mechanism of isocorydine against

Xia Wang, Weichao Ma, Yijun Yuan, Xiang Gao, Tianyu Zhao, Peng Wang, Yanmei Liu, Tingpu Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xia WangGansu Provincial Universities Key Laboratory for Agricultural Microbiology, College of Bioengineering and Biotechnology, Tianshui Normal University, Tianshui, Gansu, China.
Weichao MaGansu Provincial Universities Key Laboratory for Agricultural Microbiology, College of Bioengineering and Biotechnology, Tianshui Normal University, Tianshui, Gansu, China.
Yijun YuanGansu Provincial Universities Key Laboratory for Agricultural Microbiology, College of Bioengineering and Biotechnology, Tianshui Normal University, Tianshui, Gansu, China.
Xiang GaoGansu Provincial Universities Key Laboratory for Agricultural Microbiology, College of Bioengineering and Biotechnology, Tianshui Normal University, Tianshui, Gansu, China.
Tianyu ZhaoCollege of Chemical Engineering and Technology, Tianshui Normal University, Tianshui, Gansu, China.
Peng WangCollege of Chemical Engineering and Technology, Tianshui Normal University, Tianshui, Gansu, China.
Yanmei LiuGansu Provincial Universities Key Laboratory for Agricultural Microbiology, College of Bioengineering and Biotechnology, Tianshui Normal University, Tianshui, Gansu, China.
Tingpu WangGansu Provincial Universities Key Laboratory for Agricultural Microbiology, College of Bioengineering and Biotechnology, Tianshui Normal University, Tianshui, Gansu, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Methods: The minimum inhibitory concentration (MIC) of ICD was determined. Phenotypic changes were assessed through assays measuring cell wall/membrane integrity, ion leakage, extracellular pH, total lipid content, and electron microscopy. The global response of Results: ICD exhibited potent antibacterial activity with a MIC of 400 μg/mL. It disrupted the cell wall and membrane, leading to ion leakage, pH alteration, reduced lipids, and severe ultrastructural damage. Transcriptomics revealed 66 differentially expressed genes, with significant upregulation of efflux pumps and TetR family regulators. Metabolomics identified 1,158 differential metabolites, indicating a profound metabolic rewiring characterized by depleted central carbon metabolites and accumulated fatty acids. Discussion/conclusion: Our results demonstrate that ICD exerts its antibacterial effect primarily by targeting the cell envelope, causing membrane disruption and energetic stress.

Indexed as

antibacterial mechanismefflux pumpsisocorydinemetabolic reprogrammingmetabolomicsmulti-omicsMycobacterium bovistranscriptomics

Identifiers

PMID41459214
PMCPMC12740009

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.