Evidence map›Paper›PMID 41462297›Full record

ArticleBiology direct2025

Spatiotemporal dynamics of neuronal subtypes and their interactions with glia following intracortical electrode implantation.

Zhi Zhao, Xiaoge Duan, Jiaqi Luo, Zhaoyuan He, Yuxue Zhang, Meiting Wang, Jiaoqin Qin, Sen Lin, Hailan Chen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology direct, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Zhi ZhaoGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China.
Xiaoge DuanGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China.
Jiaqi LuoGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China.
Zhaoyuan HeGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China.
Yuxue ZhangMedical School of Guangxi University, Guangxi University, Nanning, Guangxi, 530004, China.
Meiting WangMedical School of Guangxi University, Guangxi University, Nanning, Guangxi, 530004, China.
Jiaoqin QinDepartment of Neurology, Jiangbin Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, 530012, China.
Sen LinSchool of Physical Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China. slin@gxu.edu.cn.
Hailan ChenGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China. hlchen319@163.com.

Funding

National Natural Science Foundation of China 32201095
6 · The paper itself

Abstract

backgroundChronically implanted electrodes offer a promising approach for treating neurological disorders via brain-computer interfaces, yet their long-term efficacy is compromised by the neuroinflammatory foreign body response. While neurons are central to both electrode function and inflammatory regulation, their specific responses post-implantation remain poorly characterized. Here, we combined single-nucleus RNA sequencing (snRNA-seq) and immunofluorescence to delineate the spatiotemporal dynamics of neuronal subtypes in the rat motor cortex at 3, 25, and 50 days after electrode implantation.

resultsWe identified 22 distinct neuronal subpopulations, among which clusters 5, 6, and 8 emerged as injury-responsive subtypes during the acute phase (3 days), exhibiting a specific upregulation of Tmsb4x, a key regulator of neuronal plasticity and repair. Furthermore, our analysis revealed activated signaling pathways mediating neuron-glia communication, most notably the Ptn-Sdc4 and Il34/Csf1-Csf1R axes between neurons and astrocytes.

conclusionsThese findings provide a high-resolution map of neuronal adaptation to intracortical implants, uncovering previously unknown repair-associated neuronal subtypes and specific ligand-receptor pairs that coordinate the neuroinflammatory microenvironment, which offers novel insights and potential therapeutic targets for improving the biocompatibility and long-term stability of neural electrodes.

Indexed as

Electrodes, ImplantedMotor CortexNeurogliaNeuronsAnimalsMaleRatsRats, Sprague-DawleyCell-cell communicationIntracortical electrodeNeuronal subtypesSingle-nucleus RNA sequencingTmsb4x

Identifiers

PMID41462297
PMCPMC12853883

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.