Evidence mapPaperPMID 41462313Full record

ArticleJournal of orthopaedic surgery and research2025

miR-202-5p mitigates the advancement of osteoporosis through the regulation of PTEN.

Zilong Geng, Dawei He, Siyuan Ha, Jing Xiao, Ning Gan, Huan Yang, Jinxiang Zhang

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Article in Journal of orthopaedic surgery and research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Zilong Geng *Department of Orthopedics, Wuhan No.1 Hospital, Wuhan, 430022, Hubei, China.
Dawei He *Department of Orthopedics and Traumatology, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing, 102218, China.
Siyuan HaDepartment of Anesthesiology, The Wuhan Hospital of TCM Affiliated to Hubei University of Traditional Chinese Medicine, Wuhan, 430014, Hubei, China.
Jing XiaoDepartment of Endocrinology, Wuhan No.9 Hospital, Wuhan, 430000, Hubei, China.
Ning GanDepartment of Orthopedics, The Wuhan Hospital of TCM Affiliated to Hubei University of Traditional Chinese Medicine, Wuhan, 430014, Hubei, China.
Huan YangDepartment of Orthopedics, The Wuhan Hospital of TCM Affiliated to Hubei University of Traditional Chinese Medicine, Wuhan, 430014, Hubei, China.
Jinxiang ZhangDepartment of Joint Surgery and Geriatric Orthopedics, The Affiliated Hospital of Youjiang Medical University for Nationalities, No. 18, Zhongshan 2nd Road, Youjiang District, Baise, 533000, China. jxzhang12342025@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMicroRNAs play a crucial regulatory function in preserving bone remodeling balance and bone metabolic homeostasis.

aimsThis study aimed to detect miR-202-5p expression in OP patients and investigate its role and potential regulatory mechanism in OP development.

methodsqRT-PCR was used to determine miR-202-5p and PTEN expression in serum. The receiver operating characteristic curve was utilized to evaluate miR-202-5p's diagnostic value for OP. CCK-8 and flow cytometry were applied to determine the effects of miR-202-5p on the proliferation and apoptosis of human fetal osteoblasts. Luciferase reporter assay was conducted to verify the targeted binding between miR-202-5p and PTEN.

resultsmiR-202-5p in OP patients was markedly downregulated, and miR-202-5p exhibited high diagnostic value for OP. In vitro experiments showed that miR-202-5p promoted hFOB proliferation, inhibited apoptosis, and enhanced osteogenic differentiation. PTEN expression was significantly higher in OP patients. Prediction via the miRDB database indicated that PTEN might be a target gene of miR-202-5p, which was further confirmed by luciferase assay, verifying their interaction. Additionally, downregulation of PTEN significantly promoted osteogenic differentiation.

conclusionsmiR-202-5p holds promise as a novel potential diagnostic biomarker for OP. miR-202-5p may regulate osteoblast proliferation, apoptosis, and osteogenic differentiation by targeting PTEN, thereby exerting a role in OP.

Indexed as

MicroRNAsOsteoporosisPTEN PhosphohydrolaseApoptosisCell DifferentiationCell ProliferationCells, CulturedDown-RegulationFemaleHumansMaleMiddle AgedOsteoblastsOsteogenesisMicroRNAsMIRN202 microRNA, humanPTEN PhosphohydrolasePTEN protein, humanDiagnosismiR-202-5pOsteogenesisOsteoporosisPTEN

Identifiers

PMID41462313
PMCPMC12751501

What Socratic holds

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LicenceCC BY-NC-ND
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.