Evidence map›Paper›PMID 41463596›Full record

ArticleBioengineering (Basel, Switzerland)2025

SRRM4 Knockout Helps the Human Mesenchymal Stem Cell Line to Penetrate Decellularized Cancellous Bone.

Iichiroh Onishi, Karin Muraoka, Anri Koyanagi, Tsuyoshi Kimura, Akio Kishida, Masanobu Kitagawa, Morito Kurata

Abstract read
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In one paragraph

Article in Bioengineering (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Iichiroh OnishiDepartment of Pathology, Institute of Science Tokyo Hospital, 1-5-45 Yushima, Bunkyoku, Tokyo 113-8510, Japan.ORCID 0000-0001-9744-0114
Karin MuraokaDepartment of Comprehensive Pathology, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Tokyo 113-8519, Japan.
Anri KoyanagiDepartment of Pathology, Institute of Science Tokyo Hospital, 1-5-45 Yushima, Bunkyoku, Tokyo 113-8510, Japan.
Tsuyoshi KimuraDepartment of Biomedical Engineering, Faculty of Life Sciences, Toyo University, Saitama 351-8510, Japan.ORCID 0000-0003-0476-0624
Akio KishidaLaboratory for Biomaterials and Bioengineering, Institute of Integrated Research, Institute of Science Tokyo, Tokyo 101-0062, Japan.ORCID 0000-0002-2434-3564
Masanobu KitagawaDepartment of Comprehensive Pathology, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Tokyo 113-8519, Japan.
Morito KurataDepartment of Pathology, Institute of Science Tokyo Hospital, 1-5-45 Yushima, Bunkyoku, Tokyo 113-8510, Japan.ORCID 0000-0002-1242-6709

Funding

Japan Mint 21K15414
6 · The paper itself

Abstract

backgroundAnalyzing the human bone marrow microenvironment requires an in vivo model that reflects the human bone marrow microenvironment. Introducing a human bone marrow mesenchymal stem cell (MSC) line into decellularized cancellous bone (DCB) is a first step in forming such a bone marrow model. Our goal with this research is identifying factors that promote the penetration of MSCs into DCBs in an ex vivo setting.

methodsWe introduced the CRISPR Knock Out (GeCKO v2) library to identify candidate genes in UE7T-9 cell line (MSC line) for DCB penetration. We established a candidate gene-knockout UE7T-9 cell for validation and evaluated its penetration into DCB (measured distance of randomly selected 100 cells), proliferation (MTS assay), migration (scratch assay), and ancorage-independent growth (soft agar assay). RNA sequencing was performed to analyze changes in gene expression comprehensively.

resultsWe identified

conclusions

Indexed as

bone marrowbone marrow mesenchymal stem cell (MSC) lineCRISPR knock out librarydecellularized bone (DCB)Serine/Arginine Repetitive Matrix 4 (SRRM4)

Identifiers

PMID41463596
PMCPMC12729399

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.