Evidence map›Paper›PMID 41465225›Full record

ArticleInternational journal of molecular sciences2025

Innovative Colorimetric Neutral Red-Based Loop-Mediated Isothermal Amplification (NR-LAMP) Assay: Transforming Rapid and Affordable Feline Leukemia Virus Detection.

Witsanu Rapichai, Piyamat Khamsingnok, Anyalak Wachirachaikarn, Thawee Laodim, Hieu Van Dong, Nianrawan Meecharoen, Siriluk Ratanabunyong, Thanawat Khaoiam, Supansa Tuanthap, Amonpun Rattanasrisomporn and 5 more

Abstract read
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In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Witsanu RapichaiCenter for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok 10900, Thailand.ORCID 0009-0001-8529-4137
Piyamat KhamsingnokDepartment of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
Anyalak WachirachaikarnDepartment of Sciences and Bioinnovation, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
Thawee LaodimDepartment of Animal Science, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
Hieu Van DongFaculty of Veterinary Medicine, Vietnam National University of Agriculture, Trau Quy Town, Gia Lam District, Hanoi 131000, Vietnam.ORCID 0000-0002-6340-9204
Nianrawan MeecharoenCentral Laboratory (CTL), Center for Veterinary Research and Innovation, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
Siriluk RatanabunyongDepartment of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.ORCID 0000-0003-1078-186X
Thanawat KhaoiamDepartment of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
Supansa TuanthapFaculty of Veterinary Medicine, Rajamangala University of Technology Tawan-ok, Bangpra, Chonburi 20110, Thailand.
Amonpun RattanasrisompornInterdisciplinary of Genetic Engineering and Bioinformatics, Graduate School, Kasetsart University, Bangkok 10900, Thailand.
Selapoom PairorDepartment of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
Kiattawee ChoowongkomonDepartment of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.ORCID 0000-0002-2421-7859
Natthasit TansakulDepartment of Pharmacology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.ORCID 0000-0002-2991-2682
Peter A LieberzeitDepartment of Physical Chemistry, Faculty of Chemistry, University of Vienna, Waehringer Strasse 42, A-1090 Vienna, Austria.ORCID 0000-0003-1596-0584
Jatuporn RattanasrisompornCenter for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok 10900, Thailand.ORCID 0000-0002-2426-0961

Funding

Kasetsart University Institute for Advanced Studies (KUIAS) through the Reinventing University Program RUP69_Act2_No.17 (PD)Kasetsart University Research and Development Institute, KURDI FF(KU-SRIU)7.68the Faculty of Veterinary Medicine, Kasetsart University
6 · The paper itself

Abstract

Feline leukemia virus (FeLV) is a retrovirus that globally affects both domestic and wild cats, leading to the development of leukemia, lymphoma, and immunosuppression. However, it is important to note that the daily antigen test may yield false negative results. In this study, we successfully developed the first colorimetric loop-mediated isothermal amplification (LAMP) associated with neutral red (NR-LAMP) for the detection of FeLV. The NR-LAMP assay exhibited high sensitivity and efficiency compared to the routine polymerase chain reaction (PCR) reference method. To ensure specificity, a novel LAMP primer set was custom-designed based on the

Indexed as

ColorimetryLeukemia, FelineLeukemia Virus, FelineMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesAnimalsCatsLimit of DetectionSensitivity and Specificitycolorimetric LAMPcost-effective clinical applicationfeline leukemia virus (FeLV)neutral redpol gene

Identifiers

PMID41465225
PMCPMC12733238

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.