Evidence mapPaperPMID 41465331Full record

ArticleInternational journal of molecular sciences2025

Type-1 Cannabinoid Receptor Promiscuous Coupling: Computational Insights into Receptor-G Protein Interaction Dynamics.

Alessandro Berghella, Tomasz Maciej Stepniewski, Annalaura Sabatucci, Marta Lopez-Balastegui, Krzysztof Nowicki, Beatrice Dufrusine, Jana Selent, Enrico Dainese

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Alessandro BerghellaDepartment of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64100 Teramo, Italy.ORCID 0000-0002-1743-6404
Tomasz Maciej StepniewskiResearch Program on Biomedical Informatics, Hospital del Mar Research Institute, 08003 Barcelona, Spain.ORCID 0000-0002-6828-9192
Annalaura SabatucciDepartment of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64100 Teramo, Italy.ORCID 0000-0002-8004-2547
Marta Lopez-BalasteguiResearch Program on Biomedical Informatics, Hospital del Mar Research Institute, 08003 Barcelona, Spain.ORCID 0000-0002-6283-601X
Krzysztof NowickiFaculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, Poland.ORCID 0000-0002-6887-5386
Beatrice DufrusineDepartment of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64100 Teramo, Italy.ORCID 0000-0001-7515-9794
Jana SelentResearch Program on Biomedical Informatics, Hospital del Mar Research Institute, 08003 Barcelona, Spain.ORCID 0000-0002-1844-4449
Enrico DaineseDepartment of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64100 Teramo, Italy.ORCID 0000-0001-7163-9344

Funding

European Union - Next Generation EU C43C22000380007
6 · The paper itself

Abstract

Cannabinoid receptor (CB1), a G protein coupled receptor (GPCR), is a known pharmacological target in several diseases and modulates key physiological processes through Gi protein-mediated signaling. However, recent evidence suggests that CB1 can also activate other G proteins, including the stimulatory Gs protein, a phenomenon with unclear structural determinants. Here, we use a computational approach to elucidate the structural basis of the CB1-Gs interaction. Protein-protein docking and extensive molecular dynamics simulations yield a model for the CB1-Gs complex that agrees well with both existing experimental data and available GPCR-Gs structures, supporting its validity. This work provides new insights into the structural basis of CB1's ability to couple with different G-proteins. The model provides a basis for future studies dissecting the functional consequences of CB1-Gs signaling and the development of improved therapeutics targeting the CB1 receptor and the wider endocannabinoid system.

Indexed as

GTP-Binding ProteinsReceptor, Cannabinoid, CB1HumansMolecular Docking SimulationMolecular Dynamics SimulationProtein BindingSignal TransductionGTP-Binding ProteinsReceptor, Cannabinoid, CB1CB1 receptorendocannabinoid systemGPCRGs proteinmolecular dynamics

Identifiers

PMID41465331
PMCPMC12732796

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.