Evidence map›Paper›PMID 41465333›Full record

ArticleInternational journal of molecular sciences2025

Disturbances in Mitochondrial Network, Biogenesis, and Mitochondria-Mediated Inflammatory Responses in Selected Brain Structures of Rats Exposed to Lead (Pb) During Prenatal and Neonatal Development.

Mikołaj Chlubek, Magdalena Gąssowska-Dobrowolska, Agnieszka Kolasa, Maciej Tarnowski, Patrycja Tomasiak, Agnieszka Maruszewska, Katarzyna Barczak, Irena Baranowska-Bosiacka

Abstract read
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In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mikołaj ChlubekDepartment of Biochemistry and Medical Chemistry, Pomeranian Medical University, Powstańców Wlkp. 72, 70-111 Szczecin, Poland.ORCID 0000-0003-4301-6195
Magdalena Gąssowska-DobrowolskaDepartment of Cellular Signalling, Mossakowski Medical Research Institute, Polish Academy of Sciences, 02-106 Warsaw, Poland.ORCID 0000-0001-9777-1378
Agnieszka KolasaDepartment of Histology and Embryology, Pomeranian Medical University, Powstańców Wlkp. 72, 70-111 Szczecin, Poland.ORCID 0000-0002-6933-7643
Maciej TarnowskiDepartment of Physiology in Health Sciences, Pomeranian Medical University, Żołnierska 54, 70-210 Szczecin, Poland.ORCID 0000-0002-3869-5077
Patrycja TomasiakInstitute of Physical Culture Sciences, University of Szczecin, 70-240 Szczecin, Poland.ORCID 0000-0002-8147-6419
Agnieszka MaruszewskaInstitute of Biology, University of Szczecin, Wąska 13, 71-415 Szczecin, Poland.ORCID 0000-0003-0687-9717
Katarzyna BarczakDepartment of Endodontic Surgery, Pomeranian Medical University, Powstańców Wlkp. 72, 70-111 Szczecin, Poland.ORCID 0000-0001-6381-566X
Irena Baranowska-BosiackaDepartment of Biochemistry and Medical Chemistry, Pomeranian Medical University, Powstańców Wlkp. 72, 70-111 Szczecin, Poland.ORCID 0000-0001-9187-8895

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lead (Pb) disrupts mitochondrial function, but its impact on the mitochondrial dynamics and biogenesis during early brain development remains insufficiently understood. This study aimed to investigate the effects of pre- and neonatal Pb exposure on the processes involved in mitochondrial network formation in the brains of rat offspring, simulating environmental exposure. We quantified mRNA expression (qRT-PCR) and protein levels (ELISA) of key mitochondrial fusion (Mfn1, Mfn2, Opa1), fission (Drp1, Fis1) regulators, as well as biogenesis markers (PGC-1α, TFAM, NRF1) in the hippocampus, forebrain cortex, and cerebellum of rats exposed to Pb. Mitochondrial ultrastructure was evaluated using transmission electron microscopy (TEM), and the expression of mitochondrial electron transport chain (ETC) genes was analysed (qRT-PCR). Furthermore, to examine the involvement of the cGAS-STING pathway in Pb-induced neuroinflammation, we measured the expression of ISGs (qRT-PCR), TBK1 phosphorylation (Western blot), and 2',3'-cGAMP synthesis (ELISA). Our results showed that Pb exposure markedly reduced PGC-1α and region-specific NRF1 levels, broadly supressed fusion proteins (Mfn1, Mfn2, Opa1), increased Fis1, and depleted Drp1. ETC gene expression (

Indexed as

BrainLeadMitochondriaOrganelle BiogenesisPrenatal Exposure Delayed EffectsAnimalsAnimals, NewbornFemaleInflammationMaleMitochondrial DynamicsMitochondrial ProteinsPregnancyRatsLeadMitochondrial ProteinscGAS-STING pathwaydevelopmental lead exposurelead neurotoxicitymitochondrial biogenesismitochondrial fission/fusionrat brain

Identifiers

PMID41465333
PMCPMC12733045

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.