Evidence map›Paper›PMID 41467113›Full record

ReviewMolecular therapy. Nucleic acids2025

MicroRNA chemical modifications in post-transcriptional gene silencing and human diseases.

Amin Alaei, Pavan Kumar Kakumani

Abstract readReview
In one paragraph

Review in Molecular therapy. Nucleic acids, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Review
  5. Review
  6. Review
  7. Review
  8. Article
  9. Integrative analysis ofFrontiers in plant science · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Amin AlaeiDepartment of Biochemistry, Memorial University of Newfoundland, 45 Arctic Avenue, St. John's, NL A1C 5S7, Canada.
Pavan Kumar KakumaniDepartment of Biochemistry, Memorial University of Newfoundland, 45 Arctic Avenue, St. John's, NL A1C 5S7, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MicroRNAs (miRNAs) are small, non-coding RNAs that influence various cellular activities through post-transcriptional gene silencing. Recent research has shown that miRNA modifications, including N6-methyladenosine (m6A), 5-methylcytidine (m5C), 2'-O-methylation (Nm), N7-methylguanosine (m7G), pseudouridylation (Ψ), phosphorylation, RNA editing (adenosine to inosine [A to I]), acetylation, and oxidation, play crucial roles in fine-tuning miRNA expression and function. This review examines the impact of nucleotide modifications on miRNA biogenesis, particularly their role in regulating RNA interactions with the Drosha-DiGeorge syndrome critical region 8 (DGCR8) and Dicer complexes, thereby influencing primary miRNA (pri-miRNA) processing, pre-miRNA export, and miRNA maturation. It also examines whether these modifications assist miRNA recognition by RNA-binding proteins (RBPs) in controlling miRNA processing and stability, as well as their impact on miRNA strand selection, target recognition, and the recruitment of regulatory proteins to the miRNA-induced silencing complex (miRISC), which facilitates the silencing of miRNA-targeted messenger RNAs (mRNAs). Additionally, the review discusses the role of miRNA modifications in various human diseases and considers how advanced sequencing technologies and chemical biology approaches enable detailed mapping of these modifications. Furthermore, it provides new insights into the challenges of understanding the dynamic nature of miRNA modifications and their context-dependent effects. It also highlights future directions, including innovative detection methods and epigenetic crosstalk with potential therapeutic applications in human diseases.

Indexed as

chemical modificationsgene regulationhuman diseasesmiRNAsMT: Non-coding RNAs

Identifiers

PMID41467113
PMCPMC12744855

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.