Evidence map›Paper›PMID 41474973›Full record

ArticleThe Journal of experimental medicine2026

FOXO1 re-expression with a dual-recombinase allele rescues class switching in germinal center B cells.

Carlota Farré Díaz, Eleni Kabrani, Wiebke Winkler, Eric Blanc, Brigitte Wollert-Wulf, Claudia Salomon, F Thomas Wunderlich, Dieter Beule, Martin Janz, Klaus Rajewsky

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of experimental medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Carlota Farré Díaz *Immune Regulation and Cancer, Max Delbrück Center for Molecular Medicine in the Helmholtz Association , Berlin, Germany.ORCID 0000-0003-4306-2108
Eleni Kabrani *Immune Regulation and Cancer, Max Delbrück Center for Molecular Medicine in the Helmholtz Association , Berlin, Germany.ORCID 0000-0003-0834-9912
Wiebke WinklerImmune Regulation and Cancer, Max Delbrück Center for Molecular Medicine in the Helmholtz Association , Berlin, Germany.ORCID 0000-0001-8807-9259
Eric BlancCore Unit Bioinformatics, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin and Berlin Institute of Health , Berlin, Germany.ORCID 0000-0002-4369-0254
Brigitte Wollert-WulfBiology of Malignant Lymphomas, Max Delbrück Center for Molecular Medicine in the Helmholtz Association , Berlin, Germany.ORCID 0009-0000-0257-4049
Claudia SalomonImmune Regulation and Cancer, Max Delbrück Center for Molecular Medicine in the Helmholtz Association , Berlin, Germany.ORCID 0009-0000-6723-6497
F Thomas WunderlichFaculty of Medicine and University Hospital Cologne, Center for Molecular Medicine Cologne, University of Cologne , Cologne, Germany.ORCID 0000-0002-9209-3501
Dieter BeuleCore Unit Bioinformatics, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin and Berlin Institute of Health , Berlin, Germany.ORCID 0000-0002-3284-0632
Martin JanzBiology of Malignant Lymphomas, Max Delbrück Center for Molecular Medicine in the Helmholtz Association , Berlin, Germany.ORCID 0000-0002-1127-0044
Klaus RajewskyImmune Regulation and Cancer, Max Delbrück Center for Molecular Medicine in the Helmholtz Association , Berlin, Germany.ORCID 0000-0002-6633-6370

Funding

Berlin School of Integrative OncologyDeutsche Krebshilfe 70112800European Research Council 268921Max-Delbrück-Centrum für Molekulare Medizin in der Helmholtz-Gemeinschaft
6 · The paper itself

Abstract

Modeling complex (patho)physiological processes by sequential targeted mutagenesis in mice is limited by the lack of precision of cellular targeting and complex breeding strategies. We present a new Cre/DreERT2 dual-recombinase germinal center B cell (GCBC)-specific strain, with co-expression of the recombinases from a single allele. This enables highly efficient Cre-mediated FOXO1 knockout in GCBCs in vivo, followed by time-controlled, efficient Dre-mediated FOXO1 re-expression in the same cells, leading to functional rescue of GC compartmentalization and class switch recombination. The present approach can be easily adapted to other cellular contexts.

Indexed as

B-LymphocytesForkhead Box Protein O1Forkhead Transcription FactorsGerminal CenterImmunoglobulin Class SwitchingIntegrasesAllelesAnimalsMiceMice, Inbred C57BLMice, KnockoutCre recombinaseForkhead Box Protein O1Forkhead Transcription FactorsFoxo1 protein, mouseIntegrases

Identifiers

PMID41474973
PMCPMC12755864

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.