Evidence map›Paper›PMID 41476231›Full record

ArticleJournal of orthopaedic surgery and research2025

The functional mechanism and clinical significance of miR-3651 in the healing process of Pilon fractures.

Shuang Liang, Zhijian Sun, Tenglong Dai, Xiangyu Cao, Boxuan Huang, Yuqing Wang, Ruixuan Wang, Jun Wu

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In one paragraph

Article in Journal of orthopaedic surgery and research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Platelet-rich plasma and stem cell therapies for spondylosis: a systematic review of randomized controlled trials.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Shuang Liang *Department of Clinical Laboratory, Beijing Jishuitan Hospital, Capital Medical University, No. 31, East Xinjiekou Street, Xicheng District, Beijing, 100035, China.
Zhijian Sun *Department of Orthopaedic Trauma, Beijing Jishuitan Hospital, Capital Medical University, Beijing, 100035, China.
Tenglong DaiDepartment of Clinical Laboratory, Beijing Jishuitan Hospital, Capital Medical University, No. 31, East Xinjiekou Street, Xicheng District, Beijing, 100035, China.
Xiangyu CaoDepartment of Clinical Laboratory, Beijing Jishuitan Hospital, Capital Medical University, No. 31, East Xinjiekou Street, Xicheng District, Beijing, 100035, China.
Boxuan HuangDepartment of Orthopaedic Trauma, Beijing Jishuitan Hospital, Capital Medical University, Beijing, 100035, China.
Yuqing WangDepartment of Clinical Laboratory, Beijing Jishuitan Hospital, Capital Medical University, No. 31, East Xinjiekou Street, Xicheng District, Beijing, 100035, China.
Ruixuan WangDepartment of Clinical Laboratory, Beijing Jishuitan Hospital, Capital Medical University, No. 31, East Xinjiekou Street, Xicheng District, Beijing, 100035, China.
Jun WuDepartment of Clinical Laboratory, Beijing Jishuitan Hospital, Capital Medical University, No. 31, East Xinjiekou Street, Xicheng District, Beijing, 100035, China. junwuwj1@163.com.

Funding

Beijing Natural Science Foundation L254086, L246006
6 · The paper itself

Abstract

backgroundPilon fractures are high-energy injuries often associated with delayed healing after treatment. MiR-3651 is a short non-coding RNA, yet its expression level, therapeutic effect, and specific mechanism in the healing process of Pilon fractures remain unclear.

aimThis study investigates the association between miR-3651 expression and Pilon fracture healing, assesses its diagnostic utility, and elucidates its regulatory mechanism.

methodsThe expression levels of miR-3651, KRAS, and osteoclast differentiation markers were detected using RT-qPCR. The diagnostic efficacy of miR-3651 for delayed healing of Pilon fractures was assessed via ROC curve analysis, and logistic regression was employed to identify risk factors influencing delayed healing. Cell viability and apoptosis were measured using the CCK-8 assay and flow cytometry. The targeting relationship between miR-3651 and KRAS was verified through the dual-luciferase reporter assay.

resultsMiR-3651 expression was significantly upregulated in the delayed healing group, with concomitant downregulation of its downstream target KRAS. MiR-3651 effectively distinguished between normal healing and delayed healing and was identified as a risk factor influencing the occurrence of delayed healing. Inhibition of miR-3651 expression reduced osteoclast activity and differentiation while promoting apoptosis; conversely, overexpression of miR-3651 produced the opposite effects. Knockdown of KRAS expression reversed the impact of miR-3651 inhibition on osteoclast activity, apoptosis, and differentiation.

conclusionsDownregulation of miR-3651 expression inhibits osteoclast formation, and knockdown of KRAS restores this inhibitory effect.

Indexed as

Fracture HealingMicroRNAsTibial FracturesAdultApoptosisCell DifferentiationClinical RelevanceFemaleHumansMaleOsteoclastsProto-Oncogene Proteins p21(ras)KRAS protein, humanMicroRNAsProto-Oncogene Proteins p21(ras)Delayed healingFracture healingKRASMiR-3651MiRNA regulationOsteoclastPilon fracture

Identifiers

PMID41476231
PMCPMC12866041

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.