Evidence map›Paper›PMID 41484285›Full record

ArticleScientific reports2026

Immunomodulatory effects of primed amniotic fluid-derived mesenchymal stem/stromal cells with IFN-γ from unexplained recurrent miscarriage sources.

Seyed Mehdi Hoseini, Ahmad Reza Bahrami, Seyed Mehdi Kalantar, Mohammad Hasan Sheikhha, Behrouz Aflatoonian, Nasrin Ghasemi, Elham Sadat Hosseini, Maryam M Matin, Fateme Montazeri

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In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Seyed Mehdi HoseiniBiotechnology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
Ahmad Reza BahramiDepartment of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Seyed Mehdi KalantarResearch and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
Mohammad Hasan SheikhhaBiotechnology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
Behrouz AflatoonianStem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
Nasrin GhasemiAbortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
Elham Sadat HosseiniBiotechnology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
Maryam M MatinDepartment of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran. matin@um.ac.ir.
Fateme MontazeriAbortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran. montazeri@outlook.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The immunomodulatory properties of mesenchymal stem/stromal cells (MSCs) are strongly influenced by IFN-γ priming and may vary depending on their tissue of origin. This study investigates whether the immunoregulatory responses of human amniotic fluid-derived MSCs (hAF-MSCs) to IFN-γ are altered when derived from individuals with unexplained recurrent pregnancy loss (RPL). We analyzed 10 well-characterized hAF-MSC clones, equally divided into non-RPL and RPL groups, to assess IFN-γ-induced expression of immunomodulatory genes. Additionally, we evaluated the paracrine effects of MSC-conditioned medium on T regulatory (Treg) cell induction and macrophage polarization. Significant differences were observed between the two groups in the expression of immunomodulatory markers following IFN-γ priming, as well as in their capacity to induce Treg cells and modulate M1/M2 macrophage polarization. These findings support the hypothesis that dysregulated immune pathways contribute to unexplained RPL by revealing impaired IFN-γ responsiveness in hAF-MSCs from fetuses derived from couples with the history of RPL.

Indexed as

Abortion, HabitualAmniotic FluidImmunomodulationInterferon-gammaMesenchymal Stem CellsAdultCells, CulturedCulture Media, ConditionedFemaleHumansMacrophagesPregnancyT-Lymphocytes, RegulatoryCulture Media, ConditionedInterferon-gammaAmniotic fluidImmunomodulatory capacityMesenchymal stem/stromal cellsParacrine factorsRecurrent pregnancy loss

Identifiers

PMID41484285
PMCPMC12769564

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.