ArticleMicrobial cell factories2026
Enhanced L-tyrosine production in Bacillus amyloliquefaciens through alleviation of feedback Inhibition and coordinated pathway expression.
Article in Microbial cell factories, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
backgroundL-tyrosine is a commercially significant compound with broad applications in the food and pharmaceutical industries. The development of efficient microbial cell factories for its production is of great interest. This study describes the engineering of the food-safe Bacillus amyloliquefaciens as a host chassis for enhanced L-tyrosine biosynthesis.
resultsTo overcome the key regulatory constraint of L-tyrosine feedback inhibition, a mutant prephenate dehydrogenase (Eco-TyrAM53I/A354V) from Escherichia coli was introduced. This intervention increased the L-tyrosine titer to 446.32 mg/L, representing a 53% improvement over the control strain. The underlying mechanism was also investigated. Subsequent optimization of expression elements (promoter, 5′-UTR, and terminator) generated strain A8, which produced 560.57 mg/L of L-tyrosine. Additionally, the co-expression of key genes Eco-tyrAM53I/A354V and Bao-aroA significantly enhanced metabolic flux, boosting the titer to 1104.02 mg/L, a 257% increase relative to the control.
conclusionsThis work significantly improves L-tyrosine production in a food-grade B. amyloliquefaciens chassis and provides a suite of efficient genetic tools for strain development. The findings and engineered systems established here offer a robust platform for advancing high-level microbial production of L-tyrosine, with strong potential for industrial application.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.