Evidence map›Paper›PMID 41488663›Full record

ArticleFrontiers in immunology2025

Females with obesity exhibit greater influenza vaccine-induced immunity and protection than males in a mouse model.

Brian Wolfe, Saurav Pantha, Saranya Vijayakumar, Shristy Budha Magar, Tawfik Aboellail, Santosh Dhakal

Abstract read
In one paragraph

Article in Frontiers in immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Brian Wolfe *Department of Diagnostic Medicine/Pathobiology (DMP), College of Veterinary Medicine (CVM), Kansas State University (KSU), Manhattan, KS, United States.
Saurav Pantha *Department of Diagnostic Medicine/Pathobiology (DMP), College of Veterinary Medicine (CVM), Kansas State University (KSU), Manhattan, KS, United States.
Saranya VijayakumarDepartment of Diagnostic Medicine/Pathobiology (DMP), College of Veterinary Medicine (CVM), Kansas State University (KSU), Manhattan, KS, United States.
Shristy Budha MagarDepartment of Diagnostic Medicine/Pathobiology (DMP), College of Veterinary Medicine (CVM), Kansas State University (KSU), Manhattan, KS, United States.
Tawfik AboellailDepartment of Diagnostic Medicine/Pathobiology (DMP), College of Veterinary Medicine (CVM), Kansas State University (KSU), Manhattan, KS, United States.
Santosh DhakalDepartment of Diagnostic Medicine/Pathobiology (DMP), College of Veterinary Medicine (CVM), Kansas State University (KSU), Manhattan, KS, United States.

Funding

Sex-specific effects of obestity on Influenza A virus infection and immunityP20GM130448 · NIGMS · KANSAS STATE UNIVERSITY · PI WAITHAKA MWANGI · 2020 to 2026
$15.8M
NIGMS NIH HHS P20 GM130448
6 · The paper itself

Abstract

Introduction: Obesity is increasing globally, and it negatively impacts influenza vaccine efficacy. Although sex differences in influenza vaccine responses are studied in non-obese hosts, studies investigating sex differences in influenza vaccine-induced immunity and protection during obesity are limited. Materials and methods: Using the C57BL/6J mouse model of high-fat diet (HFD)-induced obesity or low-fat diet controls, we investigated sex differences in influenza vaccine-induced immunity and protection during obesity. Male and female mice with or without obesity were vaccinated intramuscularly twice at a 3-week interval with an inactivated 2009 H1N1 influenza A virus (IAV) vaccine. At 35 days post-vaccination (dpv), antibody responses in plasma and B- and T-cell responses in spleen and bone marrow were quantified. At 42 dpv, mice were intranasally challenged with a drift variant of the H1N1 IAV, and disease severity was assessed by monitoring the change in body mass up to 21 days post-challenge (dpc). Subsets of mice were euthanized at 3 dpc to determine pulmonary virus replication (TCID Results: Female mice, irrespective of diet and obesity status, developed higher antibody responses and were better protected compared to males. Vaccinated males with obesity mounted the poorest antibody responses, experienced a more severe disease, were unable to clear replicating virus from the lungs effectively, and demonstrated heightened pulmonary inflammation. Despite these differences, splenic B- and T-cell frequencies were comparable, suggesting the inefficiency of B cells to produce antibodies in males but not in females with obesity. Discussion: Our findings suggest that sex differences are observed in influenza vaccine-induced immunity and protection during obesity, where males are more severely affected. These findings highlight the importance of considering biological sex and obesity status in influenza vaccine design and testing.

Indexed as

Influenza A Virus, H1N1 SubtypeInfluenza VaccinesObesityOrthomyxoviridae InfectionsAnimalsAntibodies, ViralB-LymphocytesCytokinesDiet, High-FatDisease Models, AnimalFemaleMaleMiceMice, Inbred C57BLSex CharacteristicsSex FactorsAntibodies, ViralCytokinesInfluenza VaccinesB-cell responsesbody mass index (BMI)influenza vaccinesvaccine efficacyvirus-neutralizing antibodies

Identifiers

PMID41488663
PMCPMC12756896

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.