Evidence mapPaperPMID 41491150Full record

ArticleBiological trace element research2026

Elemental and Isotopic Profiles in Blood and Urine of Jordanian Children with Autism Spectrum Disorder: a Preliminary Comparative Study with Neurotypical Children.

Ayat Hussein B Rashaid, Tasneem Hussein Shanayneh, Rand Mohammad Jaradat, Shreen Deeb Nusair, Mahmoud Ahmad Bashtawi

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Biological trace element research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ayat Hussein B RashaidDepartment of Chemistry, Faculty of Sciences and Arts, Jordan University of Science and Technology, P.O. Box 3030, Irbid, 22110, Jordan. ahbanirashaid@just.edu.jo.
Tasneem Hussein ShanaynehDepartment of Chemistry, Faculty of Sciences and Arts, Jordan University of Science and Technology, P.O. Box 3030, Irbid, 22110, Jordan.
Rand Mohammad JaradatDepartment of Chemistry, Faculty of Sciences and Arts, Jordan University of Science and Technology, P.O. Box 3030, Irbid, 22110, Jordan.
Shreen Deeb NusairDepartment of Clinical Pharmacy, Faculty of Pharmacy, University of Science and Technology, Irbid, Jordan.
Mahmoud Ahmad BashtawiDepartment of Neuroscience, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan.

Funding

Faculty of Research, Jordan University of Science and Technology 2022/018
6 · The paper itself

Abstract

Autism Spectrum Disorder (ASD) is a neurodevelopmental condition that may involve disruptions in metal homeostasis. This study explores the novel use of stable isotope ratios as biomarkers for ASD. The isotopic compositions of essential elements (Mg, Cr, Fe, Cu, Zn, Sr) were measured in blood and urine of children with ASD and neurotypical children to predict condition severity. 38 children (20 with ASD, 18 neurotypical), aged 2–6 years were recruited. Inductively coupled plasma mass spectrometry (ICP-MS) was used to analyze elemental and isotopic concentrations. Results revealed children with ASD had substantially lower blood levels of chromium (e.g., ⁵²Cr: 7.14 × 10¹±5.93 vs. neurotypical, p = 0.004) and copper (e.g., ⁶⁵Cu: 4.63 × 10¹±5.33, p = 0.004), with large effect sizes (Cohen’s d> -0.98). These findings remained significant after false discovery rate correction. Notably, distinct isotopic fractionation was observed. The ASD group showed pronounced enrichment of heavy magnesium (²⁶Mg) in blood, with a δ-value of 6604.14 compared to 5444.84 in neurotypical. Conversely, a significant depletion of heavy zinc (⁶⁶Zn) was found in ASD blood (δ-value: 1113.45 vs. 1619.64 in neurotypical). In urine, magnesium isotopes were significantly reduced (e.g., ²⁴Mg: 3.23 × 10³±1.34 × 10³ in ASD vs. neurotypical, p = 0.001). Predictive modeling using δ-values differentiated between both groups. The isotopic ratios, particularly δ²⁶Mg/²⁴Mg in blood and δ²⁵Mg/²⁴Mg in urine, were more discriminative than absolute concentrations, highlighting their potential as sensitive biomarkers of altered metal metabolism in ASD. This study concludes that stable isotope analysis of blood and urine presents a promising tool for developing biomarkers to aid severity prediction of ASD.

Indexed as

Autism Spectrum DisorderBiomarkersChildChild, PreschoolFemaleHumansIsotopesJordanMaleMass SpectrometryBiomarkersIsotopesAutism spectrum disorderBiomarkersICP-MSMagnesium isotopesMetal metabolismStable isotope analysis

Identifiers

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.