Evidence map›Paper›PMID 41491532›Full record

ArticleMicrobiome2026

Ningxiang pig-derived Enterococcus hirae protects against E. coli-induced gut dysbiosis and inflammation via acetate/propionate-MyD88-NF-κB axis in piglets.

Longlin Zhang, Zichen Wu, Haibo Shen, Yunlong Meng, Hongkun Li, Rong Cai, Dalin Tang, Meng Kang, Yulong Yin, Bie Tan and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiome, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Longlin Zhang *College of Animal Science and Technology, Key Laboratory for Quality Regulation of Livestock and Poultry Products of Hunan Province, Hunan Agricultural University, Changsha, 410128, China.
Zichen Wu *College of Animal Science and Technology, Key Laboratory for Quality Regulation of Livestock and Poultry Products of Hunan Province, Hunan Agricultural University, Changsha, 410128, China.
Haibo Shen *College of Animal Science and Technology, Key Laboratory for Quality Regulation of Livestock and Poultry Products of Hunan Province, Hunan Agricultural University, Changsha, 410128, China.
Yunlong MengCollege of Animal Science and Technology, Key Laboratory for Quality Regulation of Livestock and Poultry Products of Hunan Province, Hunan Agricultural University, Changsha, 410128, China.
Hongkun LiCollege of Animal Science and Technology, Key Laboratory for Quality Regulation of Livestock and Poultry Products of Hunan Province, Hunan Agricultural University, Changsha, 410128, China.
Rong CaiCollege of Animal Science and Technology, Key Laboratory for Quality Regulation of Livestock and Poultry Products of Hunan Province, Hunan Agricultural University, Changsha, 410128, China.
Dalin TangCollege of Animal Science and Technology, Key Laboratory for Quality Regulation of Livestock and Poultry Products of Hunan Province, Hunan Agricultural University, Changsha, 410128, China.
Meng KangCollege of Animal Science and Technology, Key Laboratory for Quality Regulation of Livestock and Poultry Products of Hunan Province, Hunan Agricultural University, Changsha, 410128, China.
Yulong YinYuelushan Laboratory, Changsha, 410128, China.
Bie TanCollege of Animal Science and Technology, Key Laboratory for Quality Regulation of Livestock and Poultry Products of Hunan Province, Hunan Agricultural University, Changsha, 410128, China. bietan@hunau.edu.cn.
Jing WangCollege of Animal Science and Technology, Key Laboratory for Quality Regulation of Livestock and Poultry Products of Hunan Province, Hunan Agricultural University, Changsha, 410128, China. jingwang023@hunau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIntestinal inflammation, often driven by microbial dysbiosis and infections, remains a significant health challenge with limited effective treatments. Identifying probiotic strains with anti-inflammatory properties and elucidating their mechanisms is essential for developing novel therapeutic strategies. This study investigates the molecular mechanisms by which E. hirae-a lactic acid bacterium (LAB) isolated from Ningxiang piglets with low diarrhea incidence-alleviates E. coli-induced intestinal inflammation.

resultsIn the present study, comparative analysis showed that Ningxiang piglets exhibited a significantly lower incidence of diarrhea and reduced E. coli abundance compared to Yorkshire piglets. Notably, E. hirae was more abundant in Ningxiang piglets and correlated with elevated secretory IgA levels. Additionally, in vitro antagonism assays found that E. hirae effectively inhibited E. coli growth. In vivo supplementation of E. hirae in E. coli-infected piglets restored intestinal microbial balance, increased levels of short-chain fatty acids (SCFAs) such as acetate and propionate, and mitigated E. coli colonization. Further analyses suggested that acetate and propionate downregulated the MyD88/NF-κB signaling pathway, thereby reducing pro-inflammatory cytokine expression. Molecular docking and MyD88

conclusionToken together, our results reveal that Ningxiang pig-derived E. hirae alleviates E. coli-induced gut dysbiosis and inflammation potentially through the acetate/propionate-MyD88-NF-κB axis. This work provides mechanistic insights for further exploration of probiotic and postbiotic approaches against bacterial-induced intestinal inflammation. Video Abstract.

Indexed as

DysbiosisEscherichia coliEscherichia coli InfectionsGastrointestinal MicrobiomeInflammationProbioticsAcetatesAnimalsDiarrheaFatty Acids, VolatileMyeloid Differentiation Factor 88NF-kappa BPropionatesSignal TransductionSwineSwine DiseasesAcetatesFatty Acids, VolatileMyeloid Differentiation Factor 88NF-kappa BPropionatesAcetate/propionate-MyD88-NF-κB axisE. hiraeIntestinal inflammationNingxiang piglets

Identifiers

PMID41491532
PMCPMC12871038

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.