ArticleWorld journal of microbiology & biotechnology2026
Unveiling the antibacterial effects of endolysin POE and holin Hol8 from Pseudomonas otitidis phage vB_PotS-PotUPM1.
Article in World journal of microbiology & biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The global rise of antibiotic resistance poses a major threat to human health, driving the urgent search for novel antimicrobial strategies. Phage-derived proteins, such as endolysins and holins, represent promising alternatives to conventional antibiotics. Endolysins enzymatically degrade bacterial peptidoglycan but are often ineffective against Gram-negative bacteria due to the outer membrane. Holins, by disrupting cytoplasmic membranes, may bypass this barrier and provide a distinct antibacterial mechanism. In this study, a phage-derived endolysin (POE) and holin (Hol8) from Pseudomonas phage vB_PotS-PotUPM1 were cloned and expressed in Escherichia coli. Antibacterial activity against Pseudomonas otitidis NK1, an emerging zoonotic pathogen, was evaluated using well diffusion, plate lysis, turbidity reduction, and time-kill assays, with structural properties predicted through in silico analysis. POE, a globular protein with a single lysozyme-like domain, exhibited lytic activity only when the outer membrane was permeabilized, achieving a 40.4 ± 1.3% OD
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