Evidence map›Paper›PMID 41497638›Full record

ArticlebioRxiv : the preprint server for biology2025

Gut microbiota gate host exposure to cholinesterase inhibitors from dietary Solanums.

Catherine S Liou, Tijs Louwies, Mikhail Iakiviak, Jakub Rajniak, Pallavi P Murugkar, Steven K Higginbottom, Allison Weakley, Xiandong Meng, Phyu Htet, Ashley V Cabrera and 6 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Catherine S LiouDepartment of Chemical Engineering, Stanford University, Stanford, CA, USA.
Tijs LouwiesDivision of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA.
Mikhail IakiviakSarafan ChEM-H Institute, Stanford University, Stanford, CA, USA.
Jakub RajniakDepartment of Bioengineering, Stanford University, Stanford, CA, USA.
Pallavi P MurugkarSarafan ChEM-H Institute, Stanford University, Stanford, CA, USA.
Steven K HigginbottomSarafan ChEM-H Institute, Stanford University, Stanford, CA, USA.
Allison WeakleySarafan ChEM-H Institute, Stanford University, Stanford, CA, USA.
Xiandong MengSarafan ChEM-H Institute, Stanford University, Stanford, CA, USA.
Phyu HtetSarafan ChEM-H Institute, Stanford University, Stanford, CA, USA.
Ashley V CabreraSarafan ChEM-H Institute, Stanford University, Stanford, CA, USA.
Marissa JasperSarafan ChEM-H Institute, Stanford University, Stanford, CA, USA.
Alex DimasSarafan ChEM-H Institute, Stanford University, Stanford, CA, USA.
E A SchulmanHoward Hughes Medical Institute, Stanford, CA, USA.
Michael A FischbachSarafan ChEM-H Institute, Stanford University, Stanford, CA, USA.ORCID 0000-0003-3079-8247
Purna C KashyapDivision of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA.
Elizabeth S SattelyDepartment of Chemical Engineering, Stanford University, Stanford, CA, USA.ORCID 0000-0002-7352-859X

Funding

Mechanisms of alteration of GI physiology by gut microbesR01DK114007 · NIDDK · MAYO CLINIC ROCHESTER · PI Purna C Kashyap · 2017 to 2026
$4.7M
NIDDK NIH HHS R01 DK114007
6 · The paper itself

Abstract

Dietary plants are molecularly rich but the fates of these compounds post-ingestion and their implications for human health are largely unknown. Here, we systematically characterized the major chemical contributions of widely consumed Solanum species (nightshades) to the human metabolome. Using untargeted metabolomics, we found that a series of steroidal alkaloids resulting from glycoalkaloids tomatine, solanine, and chaconine are dominant diet-derived compounds in systemic circulation following ingestion of tomato and potato. By comparing serum and tissue metabolomes of colonized and microbiome-depleted mice, we determined that the gut microbiota modifies these compounds extensively, altering their absorption and gating host exposure. By screening the metabolic products in human urine and stool samples, we established that steroidal glycoalkaloid metabolism varies inter-individually in a population. Furthermore, using a collection of representative human commensal type strains, we found that a limited set of strains is responsible for steroidal glycoalkaloid metabolism, with the chemical output of a community determined by its strain-level composition. These findings enabled the rational design of complex synthetic microbial communities that controlled host exposure to steroidal alkaloid metabolites

Identifiers

PMID41497638
PMCPMC12767311

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.