Evidence map›Paper›PMID 41499415›Full record

ArticlePloS one2026

Mechanochemical interactions in cancer cells: The role of substrate stiffness in cell behavior and drug response.

Sayed Reza Ramezani, Afsaneh Mojra, Mohammad Tafazzoli-Shadpour

Abstract read
In one paragraph

Article in PloS one, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Sayed Reza RamezaniDepartment of Mechanical Engineering, K. N. Toosi University of Technology, Tehran, Iran.ORCID https://orcid.org/0009-0001-6429-6715
Afsaneh MojraDepartment of Mechanical Engineering, K. N. Toosi University of Technology, Tehran, Iran.ORCID https://orcid.org/0000-0001-8790-7016
Mohammad Tafazzoli-ShadpourDepartment of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cancer cells adhere to the extracellular matrix, where they sense and respond to variations in substrate stiffness, influencing their proliferation and invasive potential. Numerous studies have examined the biological activities of cells in relation to mechanical forces; however, research addressing the combined effects of mechanical and chemical interactions on cancer cell behavior across different metastatic stages remains limited. Moreover, the influence of chemotherapeutic drugs in the context of specific cellular characteristics remains underexplored. Therefore, in this study, synthetic polyacrylamide gels with varying elastic moduli were utilized to effectively mimic the diversity of host tissue environments for prostate cancer cells. Additionally, cellular behavior of prostate cancer cells with differing metastatic potential-low (LNCaP), medium (DU145), and high (PC3)-was evaluated in response to anticancer drugs. Ultimately, effects of drug treatment were comprehensively examined using Docetaxel, Bicalutamide, and Abiraterone Acetate, which target distinct cellular components and activate diverse signaling pathways. The assessments were based on the analysis of actin filament content and organization, size of nucleus, and cellular elastic modulus. The results revealed that a soft substrate improves the medication efficacy, resulting in an enhanced cell death rate of 40-60% compared to 20-30% on a stiff substrate. Cells cultured on soft substrates exhibited lower phalloidin content (8-16%) compared to those on stiff substrates (18-32%). Additionally, drug treatments influenced cell mechanics, with Docetaxel reducing the elastic modulus, while Bicalutamide induced an increase. Based on these findings, a treatment strategy aimed at enhancing therapeutic efficacy can be proposed.

Indexed as

Antineoplastic AgentsProstatic NeoplasmsAcrylic ResinsAnilidesCell Line, TumorCell ProliferationDocetaxelElastic ModulusHumansMaleNitrilesTosyl CompoundsAcrylic ResinsAnilidesAntineoplastic AgentsbicalutamideDocetaxelNitrilespolyacrylamide gelsTosyl Compounds

Identifiers

PMID41499415
PMCPMC12779079

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.