Evidence mapPaperPMID 41501023Full record

ArticleNature communications2026

Drug and single-cell gene expression integration identifies sensitive and resistant glioblastoma cell populations.

Robert K Suter, Anna M Jermakowicz, Rithvik Veeramachaneni, Matthew D'Antuono, Longwei Zhang, Rishika Chowdary, Simon Kaeppeli, Madison Sharp, Pravallika Palwai, Vasileios Stathias and 18 more

Abstract read
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

28 authors.

Robert K SuterDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA. RKS82@Georgetown.edu.ORCID http://orcid.org/0000-0002-3335-8426
Anna M JermakowiczDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.
Rithvik VeeramachaneniDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.
Matthew D'AntuonoDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.ORCID http://orcid.org/0000-0002-1837-7004
Longwei ZhangDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.ORCID http://orcid.org/0009-0007-1155-4171
Rishika ChowdaryDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.
Simon KaeppeliDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.ORCID http://orcid.org/0000-0003-4449-6952
Madison SharpDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.
Pravallika PalwaiDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.
Vasileios StathiasDepartment of Molecular and Cellular Pharmacology, Institute for Data Science & Computing, University of Miami Miller School of Medicine, Miami, FL, USA.
Grace BakerDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.
Luz RuizDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.
Winston WaltersDepartment of Neurological Surgery, University of Miami Miller School of Medicine, Miami, FL, USA.
Maria CeperoDepartment of Neurology, University of Miami Miller School of Medicine, Miami, FL, USA.
Danielle BurgenskeDepartment of Radiation Oncology, Mayo Clinic, Rochester, MN, USA.
Edward B ReillyAbbVie, Oncology Discovery, North Chicago, IL, USA.
Anatol OleksijewAbbVie, Oncology Discovery, North Chicago, IL, USA.
Mark G AndersonAbbVie, Oncology Discovery, North Chicago, IL, USA.
Sion Ll WilliamsSylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL, USA.
Michael E IvanSylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL, USA.ORCID http://orcid.org/0000-0002-4798-4989
Ricardo J KomotarSylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL, USA.
Macarena I De La FuenteSylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL, USA.
Gregory SteinCurtana Pharmaceuticals, Inc., Austin, TX, USA.ORCID http://orcid.org/0000-0002-3588-8834
Alexandre WojcinskiPacific Neuroscience Institute and Saint John's Cancer Institute, Providence Health System, Santa Monica, CA, USA.
Santosh KesariPacific Neuroscience Institute and Saint John's Cancer Institute, Providence Health System, Santa Monica, CA, USA.
Jann N SarkariaDepartment of Radiation Oncology, Mayo Clinic, Rochester, MN, USA.ORCID http://orcid.org/0000-0001-7489-4885
Stephan C SchürerDepartment of Molecular and Cellular Pharmacology, Institute for Data Science & Computing, University of Miami Miller School of Medicine, Miami, FL, USA.ORCID http://orcid.org/0000-0001-7180-0978
Nagi G AyadDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA. NA853@Georgetown.edu.

Funding

Tissue Culture and Biobanking Shared ResourceP30CA051008 · NCI · GEORGETOWN UNIVERSITY · 1990 to 2025
$20.3M
Tumor Biology Research ProgramP30CA240139 · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · 2025 to 2025
$3.0M
TRAINING GRANT IN TUMOR BIOLOGYT32CA009686 · NCI · GEORGETOWN UNIVERSITY · 1996 to 2025
$2.2M
Targeting Cell-Type Specific Disease Phenotypes to Promote CNS RepairRM1NS133003 · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · 2025 to 2025
$1.2M
American Cancer Society (American Cancer Society, Inc.) IRG-23-1156148-27-IRGNational Science Foundation (NSF) #2138259, #2138286, #2138307, #2137603, and #2138296.NCI NIH HHS P30 CA051008NCI NIH HHS P30 CA240139NCI NIH HHS T32 CA009686NHLBI NIH HHS U54 HL127624NINDS NIH HHS RM1 NS133003U.S. Department of Health & Human Services | NIH | National Cancer Institute (NCI) P30CA051008U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) RM1NS133003
6 · The paper itself

Abstract

Glioblastoma (GBM) remains the most common and lethal adult malignant primary brain cancer with few treatment options. A significant issue hindering GBM therapeutic development is intratumor heterogeneity and plasticity. GBM tumors contain neoplastic cells within a fluid spectrum of diverse transcriptional states. Identifying effective therapeutics requires a platform that predicts the differential sensitivity and resistance of these states to various treatments. Here, we develop scFOCAL (Single-Cell Framework for -Omics Connectivity and Analysis via L1000), to quantify the cellular drug sensitivity and resistance landscape. Using single-cell RNA sequencing of newly diagnosed and recurrent GBM tumors, we identify compounds from the LINCS L1000 database with transcriptional response signatures selectively discordant with distinct GBM cell states, and leverage this capability to predict combination synergy. We validate the significance of these findings in vitro, ex vivo, and in vivo, and identify a combination of an OLIG2 inhibitor and Depatux-M for the treatment of GBM. Our studies suggest that scFOCAL identifies cell states that are sensitive and resistant to targeted therapies in GBM using a measure of cell and drug connectivity, which can be applied to identify new synergistic combinations.

Indexed as

Antineoplastic AgentsBrain NeoplasmsDrug Resistance, NeoplasmGene Expression Regulation, NeoplasticGlioblastomaAnimalsCell Line, TumorHumansMiceOligodendrocyte Transcription Factor 2Single-Cell AnalysisSingle-Cell Gene Expression AnalysisXenograft Model Antitumor AssaysAntineoplastic AgentsOLIG2 protein, humanOligodendrocyte Transcription Factor 2

Identifiers

PMID41501023
PMCPMC12780015

What Socratic holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.