Evidence map›Paper›PMID 41505076›Full record

ReviewProbiotics and antimicrobial proteins2026

Designing the Next Generation of Endolysins: A Triple Strategy Integrating Bioinformatics Mining, Engineering Modification and Encapsulation Formulation.

Yongkang Zhang, Xinge Cui, Luwei Chai, Yangwei Pan, Ying Yang, Hongbing Liu, Xingying Mou, Tao Le

Abstract readReview
PubMed Publisher
In one paragraph

Review in Probiotics and antimicrobial proteins, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Phage-Derived Endolysins TargetingAntibiotics (Basel, Switzerland) · 2026
    Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yongkang ZhangChongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing, 401331, P. R. China.
Xinge CuiChongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing, 401331, P. R. China.
Luwei ChaiChongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing, 401331, P. R. China.
Yangwei PanChongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing, 401331, P. R. China.
Ying YangChongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing, 401331, P. R. China.
Hongbing LiuChongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing, 401331, P. R. China.
Xingying MouChongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing, 401331, P. R. China.
Tao LeChongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing, 401331, P. R. China. letao@cqnu.edu.cn.

Funding

ostgraduate Scientific Research and Innovation Project of Chongqing Municipal Education Commission, China No. CYB240233Science and Technology Research Program of Chongqing Municipal Education Commission, China No. KJQN202400552
6 · The paper itself

Abstract

The intensifying challenge of antimicrobial resistance (AMR) has outpaced traditional antibiotic discovery, leading the World Health Organization to call for the rapid exploration of alternative therapeutic options. Endolysins-phage-encoded peptidoglycan hydrolases-exhibit potent bacteriolytic activity against multidrug-resistant pathogens and show minimal propensity for resistance development. However, their clinical translation remains hindered by several critical barriers, including resource-intensive discovery processes, limited permeability through the outer membrane of Gram-negative bacteria, and various chemical, physical, and immunological challenges. In this review, we systematically summarize the fundamental characteristics of endolysins and integrate a tripartite strategy to enhance their therapeutic potential: bioinformatics-based pre-screening mining stage, mid-stage optimization stage of engineered modifications and post-enhancement stage combining encapsulation and formulation. Challenges inherent to each stage are critically analyzed, and future directions for endolysin development are discussed. Such multiple strategies provide a comprehensive roadmap for overcoming current barriers and accelerating the development of next-generation endolysin-based therapies against AMR.

Indexed as

Anti-Bacterial AgentsEndopeptidasesComputational BiologyGram-Negative BacteriaHumansProtein EngineeringAnti-Bacterial AgentsendolysinEndopeptidasesAntimicrobialBioinformaticsDeliveryEndolysinEngineeringEnzybiotics

Identifiers

PMID41505076

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.