Evidence mapPaperPMID 41507380Full record

ArticleScientific reports2026

The miR-181d-5p/SPP1 axis inhibits the migration and invasion of colorectal cancer via the RhoA patyway.

Shaohua Hou, Ting Guo, Jing Wu, Bao Yang, Jie Zhou, Liwen Qi, Hai Li

Abstract read
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Shaohua HouFirst Clinical Medical College, General Hospital of Ningxia Medical University, 804 Shengli Road, Yinchuan, 750004, China.
Ting GuoFirst Clinical Medical College, General Hospital of Ningxia Medical University, 804 Shengli Road, Yinchuan, 750004, China.
Jing WuInternal Medicine-Oncology, People's Hospital of Ningxia Hui Autonomous Region, 301 Zhengyuan North Street, Yinchuan, 750002, China.
Bao YangDepartment of Anal-Colorectal Surgery, General Hospital of Ningxia Medical University, 804 Shengli Road, Yinchuan, 750004, China.
Jie ZhouDepartment of Anal-Colorectal Surgery, General Hospital of Ningxia Medical University, 804 Shengli Road, Yinchuan, 750004, China.
Liwen QiDepartment of Anesthesia and Perioperative Medicine, General Hospital of Ningxia Medical University, 804 Shengli Road, Yinchuan, 750004, China.
Hai LiDepartment of Anal-Colorectal Surgery, General Hospital of Ningxia Medical University, 804 Shengli Road, Yinchuan, 750004, China. doctorli1126@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Colorectal cancer (CRC), as the most common malignant tumor, seriously threatens human’s life and safety. Evidence suggests that microRNAs (miRNAs) exhibit an essential role in regulating the occurrence and development of CRC, while the detailed effects and possible mechanisms remain to be further explored. In the present study, a new tumor suppressor function of miR-181d-5p was disclosed in CRC. The expression of miR-181d-5p was obviously decreased in CRC tissues and cell lines. In addition, up-regulation of miR-181d-5p suppressed cell viability, migration, invasion, and epithelial-mesenchymal transition (EMT) progress in CRC cells detected by cell transfection, CCK-8, wound healing, transwell migration and western blot assays. Moreover, secreted phosphoprotein 1 (SPP1) was verified as a direct target of miR-181d-5p by binding its 3’-UTR, and confirmed by dual-luciferase reporter assay. Additionally, SPP1 was remarkably increased in CRC tissues and CC cell lines, and was negatively associated with miR-181d-5p expression. Furthermore, over-expression of SPP1 partially restored the inhibitory effects of up-regulated miR-181d-5p on cell viability, migration and EMT progress in CRC cells by modulating RhoA pathway. Finally, up-regulated miR-181d-5p inhibited the tumorigenesis in nude mice by regulating SPP1 mediated with RhoA pathway. To conclude, miR-181d-5p might serve as an effective target for the treatment of patients with CRC.

Indexed as

Colorectal NeoplasmsMicroRNAsrhoA GTP-Binding ProteinAnimalsCell Line, TumorCell MovementCell ProliferationCell SurvivalEpithelial-Mesenchymal TransitionFemaleGene Expression Regulation, NeoplasticHumansMaleMiceMice, NudeNeoplasm InvasivenessMicroRNAsMIrn181 microRNA, humanrhoA GTP-Binding ProteinRHOA protein, humanColorectal cancermiR-181d-5pSecreted phosphoprotein 1

Identifiers

PMID41507380
PMCPMC12873130

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.