Evidence map›Paper›PMID 41508017›Full record

ArticleBMC veterinary research2026

Astragalus polysaccharide improves canine sperm quality after chilled storage via promoting AMPK phosphorylation.

Xiaogang Huang, Zhihong Zhao, Ronggen Wang, Ruiyan Liu, Miaorong Huang, Li Li, Minhua Hu, Hengxi Wei, Shouquan Zhang

Abstract read
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In one paragraph

Article in BMC veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Xiaogang Huang *State Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agroanimal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.
Zhihong Zhao *Guangzhou General Pharmaceutical Research Institute Co., Ltd. (National Canine Laboratory Animal Resources Center), Guangzhou, 510240, China.
Ronggen WangGuangzhou General Pharmaceutical Research Institute Co., Ltd. (National Canine Laboratory Animal Resources Center), Guangzhou, 510240, China.
Ruiyan LiuGuangzhou General Pharmaceutical Research Institute Co., Ltd. (National Canine Laboratory Animal Resources Center), Guangzhou, 510240, China.
Miaorong HuangNational Regional Gene Bank of Livestock and Poultry, Gene Bank of Guangdong Livestock and Poultry, Guangzhou, 510642, China.
Li LiState Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agroanimal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.
Minhua HuGuangzhou General Pharmaceutical Research Institute Co., Ltd. (National Canine Laboratory Animal Resources Center), Guangzhou, 510240, China. myemail-cony@163.com.
Hengxi WeiState Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agroanimal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China. weihengxi@scau.edu.cn.
Shouquan ZhangState Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agroanimal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China. sqzhang@scau.edu.cn.

Funding

Key Realm R&D Program of GuangDong Province 2022B0202110002National Key Research and Development Program of China 2022YFF0710700National Natural Science Foundation of China 32272873State Key Laboratory of Swine and Poultry Breeding Industry research project ZQQZ-10
6 · The paper itself

Abstract

backgroundIn vitro semen preservation is a crucial technique for conserving genetic material from male dogs. However, during the storage, oxidative damage can compromise key sperm structures, resulting in the decline of sperm viability, affecting their recognition of oocytes, and even causing sperm death. Therefore, the addition of exogenous antioxidants to alleviate the oxidative damage to sperm has become an important strategy to improve the effect of sperm storage in vitro. Astragalus polysaccharide (APS) is a kind of water soluble heteropolysaccharide with high-efficiency antioxidant activity, but its effect and mechanism in the preservation of canine semen remain to be studied. Thus, the present was designed to investigate the effects of APS on canine sperm after chilled storage, as well as its mechanism.

resultsIt was observed that the supplementation of 0.50 mg/mL APS significantly improved sperm motility parameters, plasma membrane integrity, acrosome integrity, mitochondrial membrane potential, enhanced sperm antioxidant capacity, and promoted AMPK phosphorylation, while reduced the level of lipid peroxidation, after chilled storage. In addition, the effects of APS were partly counteracted when oxidative stressed sperm induced by H

conclusionThe supplementation of APS to the extender protects canine sperm after chilled storage via promoting AMPK phosphorylation for enhancing the antioxidant capacity of sperm. These findings provide novel insight into the mechanism of APS in protecting canine sperm after chilled storage, and reveal the feasibility of APS supplementation to sperm extender for canine sperm preservation.

Indexed as

AMP-Activated Protein KinasesAstragalus PlantPolysaccharidesSemen PreservationSpermatozoaAnimalsAntioxidantsDogsMaleMembrane Potential, MitochondrialPhosphorylationSemen AnalysisSperm MotilityAMP-Activated Protein KinasesAntioxidantsPolysaccharidesAMPK phosphorylationAstragalus polysaccharide (APS)Canine spermChilled storage

Identifiers

PMID41508017
PMCPMC12908368

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.