Evidence map›Paper›PMID 41510259›Full record

ArticleResearch square2025

Low Inducer Concentrations at 10°C Promotes Soluble Recombinant Expression of Aedes aegypti Mosquito Midgut Proteases in E. coli.

Daniel Fong, Neomi Millan, My Anh Le, Elizabeth Moreno-Galvez, Kevin Derisier, Abigail G Ramirez, Kaelyn Pluta, Kimberly Houghton, Alberto A Rascón

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Daniel FongArizona State University.
Neomi MillanSan Jose State University.
My Anh LeArizona State University.
Elizabeth Moreno-GalvezSan Jose State University.
Kevin DerisierSan Jose State University.
Abigail G RamirezArizona State University.
Kaelyn PlutaArizona State University.
Kimberly HoughtonSan Jose State University.
Alberto A RascónArizona State University.

Funding

Vector Control Strategy Through Inhibition of Aedes aegypti Midgut ProteasesSC3GM116681 · NIGMS · SAN JOSE STATE UNIVERSITY · PI RASCON, ALBERTO A · 2016 to 2023
$847k
NIGMS NIH HHS SC3 GM116681
6 · The paper itself

Abstract

Background: Soluble recombinant expression of Results: For this study, the focus was on Conclusions: Low IPTG at 10°C slows the rate of transcription/translation of recombinantly expressed mosquito proteases in bacteria. By preventing rapid accumulation in the cell, prevents aggregation, and ultimately inclusion body formation. Betaine works better at higher IPTG concentrations, but more studies are needed to better understand how this osmolyte stabilizes proteins during recombinant bacterial expression. Nonetheless, this study provides a blueprint for researchers who have never attempted IPTG concentrations > 0.1 mM to recombinantly express proteins in bacteria.

Indexed as

Aedes aegyptiBetaineDisulfide bondEscherichia coliIPTGMidgutProteasesRecombinant proteinSoluble expression

Identifiers

PMID41510259
PMCPMC12776528

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.