Evidence map›Paper›PMID 41511311›Full record

ReviewCells2025

Extracellular Vesicles in Osteogenesis: Comparative Analysis of Stem Cell Sources, Conditioning Strategies, and In Vitro Models Toward Advanced Bone Regeneration.

Luca Dalle Carbonare, Arianna Minoia, Michele Braggio, Francesca Cristiana Piritore, Anna Vareschi, Mattia Cominacini, Alberto Gandini, Franco Antoniazzi, Daping Cui, Maria Grazia Romanelli and 1 more

Abstract readReviewComparative Study
In one paragraph

Review in Cells, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Review
  3. [Preservation of necrotic bone structures: an alternative to osteonecrosis of femoral head replacement therapy].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2026
    Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Luca Dalle CarbonareDepartment of Engineering for the Innovation Medicine, University of Verona, 37100 Verona, Italy.ORCID 0000-0003-3263-6671
Arianna MinoiaDepartment of Engineering for the Innovation Medicine, University of Verona, 37100 Verona, Italy.ORCID 0000-0003-4610-1168
Michele BraggioDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, 37100 Verona, Italy.ORCID 0000-0001-7163-6194
Francesca Cristiana PiritoreDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, 37100 Verona, Italy.ORCID 0009-0003-2096-9441
Anna VareschiDepartment of Engineering for the Innovation Medicine, University of Verona, 37100 Verona, Italy.
Mattia CominaciniDepartment of Engineering for the Innovation Medicine, University of Verona, 37100 Verona, Italy.ORCID 0000-0002-4315-0282
Alberto GandiniDepartment of Surgery, Dentistry, Paediatrics and Gynaecology, University of Verona, 37134 Verona, Italy.
Franco AntoniazziDepartment of Surgery, Dentistry, Paediatrics and Gynaecology, University of Verona, 37134 Verona, Italy.ORCID 0000-0003-1405-1792
Daping CuiDepartment of Orthopedics, Shenzhen Bao'an District Central Hospital, Shenzhen 518100, China.
Maria Grazia RomanelliDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, 37100 Verona, Italy.ORCID 0000-0002-7360-1195
Maria Teresa ValentiDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, 37100 Verona, Italy.ORCID 0000-0003-1166-8033

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extracellular vesicles (EVs) derived from stem cells have emerged as promising mediators of osteogenesis, suggesting cell-free alternatives for bone tissue engineering and regenerative medicine. This review provides a comprehensive analysis of the main stem cell sources used for EV production, including bone marrow mesenchymal stem cells (BM-MSCs), adipose-derived stem cells (ADSCs), umbilical cord MSCs (UC-MSCs), induced pluripotent stem cells (iPSCs), and alternative stromal populations. Particular attention is given to the ways in which different conditioning and differentiation strategies, such as osteogenic induction, hypoxia, and mechanical stimulation, modulate EV cargo composition and enhance their therapeutic potential. We further discuss the in vitro models employed to evaluate EV-mediated bone regeneration, ranging from 2D cultures to complex 3D spheroids, scaffold-based systems, and bone organoids. Overall, this review emphasizes the current challenges related to standardization, scalable production, and clinical translation. It also outlines future directions, including bioengineering approaches, advanced preclinical models, and the integration of multi-omics approaches and artificial intelligence to optimize EV-based therapies. By integrating current knowledge, this work aims to guide researchers toward more consistent and physiologically relevant strategies to harness EVs for effective bone regeneration. Finally, this work uniquely integrates a comparative analysis of EVs from multiple stem cell sources with engineering strategies and emerging clinical perspectives, thereby providing an updated and translational framework for their application in bone regeneration.

Indexed as

Bone RegenerationExtracellular VesiclesModels, BiologicalOsteogenesisStem CellsAnimalsCell DifferentiationHumansMesenchymal Stem CellsTissue Engineeringboneextracellular vesiclesmesenchymal stem cellstissue regeneration

Identifiers

PMID41511311
PMCPMC12786284

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.