Evidence map›Paper›PMID 41511636›Full record

ArticleDiscover oncology2026

Zerumbone mediated CD1d inhibition suppresses epithelial to mesenchymal transition in triple negative breast cancer.

Ritis Kumar Shyanti, Sudhanshu Sharma, Mazharul Haque, Rajan Kumar Tiwari, Rajesh Singh, Manoj Mishra

Abstract read
In one paragraph

Article in Discover oncology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ritis Kumar ShyantiDepartment of Biological Sciences, Cancer Research Center, Alabama State University, Montgomery, AL, 36104, USA.
Sudhanshu SharmaDepartment of Biological Sciences, Cancer Research Center, Alabama State University, Montgomery, AL, 36104, USA.
Mazharul HaqueDepartment of Biological Sciences, Cancer Research Center, Alabama State University, Montgomery, AL, 36104, USA.
Rajan Kumar TiwariDepartment of Biological Sciences, Cancer Research Center, Alabama State University, Montgomery, AL, 36104, USA.
Rajesh SinghMicrobiology, Biochemistry, and Immunology, Morehouse School of Medicine, Cancer Health Equity Institute, Atlanta, GA, USA.
Manoj MishraDepartment of Biological Sciences, Cancer Research Center, Alabama State University, Montgomery, AL, 36104, USA. mmishra@alasu.edu.

Funding

Alabama State University-Auburn University Partnership to Promote Diversity in Aging ResearchR25AG070244 · NIA · ALABAMA STATE UNIVERSITY · PI MISHRA, MANOJ K., SUPPIRAMANIAM, VISHNU D · 2022 to 2024
$876k
American Cancer Society DICRIDG-22-1037199-01NIA NIH HHS R25 AG070244NIH HHS R25AG070244
6 · The paper itself

Abstract

Triple-negative breast cancer (TNBC) is characterized by aggressive metastatic behavior driven through epithelial-to-mesenchymal transition (EMT). CD1d, a non-classical MHC molecule, modulates immune responses via Natural Killer T (NKT) cells and is implicated in tumor progression. This study examines the effect of CD1d inhibition on EMT in TNBC. We demonstrate that Zerumbone (ZER, 20 µM), a phytochemical, downregulates CD1d, reducing EMT markers, cell migration, and adhesion in MDA-MB-468 and MDA-MB-231 TNBC cell lines. Conversely, CD1d upregulation by α-galactosylceramide (AGC, 2 µM) and thymosin α-1 (TA, 2 µM) promotes EMT, enhances migration and adhesion. Immunofluorescence and Western blot analyses confirmed ZER suppresses CD1d, vimentin, N-cadherin, and FASN, while increasing E-cadherin, indicating mesenchymal-to-epithelial transition (MET). AGC and TA elevate CD1d and EMT markers. Molecular docking and dynamics simulations show ZER’s stable binding to CD1d (-8.13 kcal/mol), supporting the in vitro data and it is predicted to act as a potential CD1d antagonist. These findings suggest that CD1d inhibition may represent a potential strategy to attenuate EMT associated phenotypes in TNBC.

Indexed as

Alpha-galactosylceramideCD1dEMTMolecular docking and simulationTriple-negative breast cancerZerumbone

Identifiers

PMID41511636
PMCPMC12881234

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.