ArticleJournal of endocrinological investigation2026
Caloric restriction attenuates oxidative and inflammatory markers of aging, while compromising germinative epithelium homeostasis in the rat aged testis.
Article in Journal of endocrinological investigation, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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Who cites it
2 citing papers in PubMed.
- Inflammaging: Immune-Metabolic Crosstalk Between the Prostate-Testis and Musculoskeletal System.International journal of molecular sciences · 2026Review
- The EV-mitochondrial outsourcing network as a therapeutic target for age-related testosterone deficiency: from network collapse to clinical intervention.Frontiers in endocrinology · 2026Review
Corrections and comments
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Authors and funding
12 authors.
Funding
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Abstract
purposeAging is accompanied by a chronic low-grade systemic inflammatory state known as "inflammaging", driven by the accumulation of pro-inflammatory and immunosenescent cells, oxidative stress, and dysregulation of apoptosis and autophagy. Experimental evidence suggests that caloric restriction (CR) can slow down inflammaging, although its impact on spermatogenesis remains controversial. This study aimed to investigate the effects of CR on age-related testicular inflammation and any effects on germ cells.
methodsFourteen 18-month-old Sprague-Dawley rats were randomly divided into two groups and sacrificed after 6 months: 7 rats on a normal ad libitum diet (ND) and 7 rats on CR (40% reduction in caloric intake). Western blot (WB) and immunohistochemical (IHC) analyses were performed to assess the expression of key markers of inflammation, oxidative stress, apoptosis, and autophagy, alongside morphological evaluations.
resultsIHC analysis reveals that CR disrupts germinative epithelium and somatic cells, and reduces the expression of Ob-R, GLP-1R, GPX4, NOX4, and SIRT1. WB analysis showed that CR lowered COX2 expression, through downregulation of the NFκB/MAPK pathway, decreased the expression of NLRP3 inflammasome, Caspase-1, and pro-inflammatory cytokine IL1-β, and restored redox homeostasis. Consistently, IHC highlighted a marked reduction in proinflammatory immune cell infiltration in the testis of CR rats. Moreover, as a consequence of the germ cells number reduction CR-induced, we observed a decreased expression of p75NTR and its pro-apoptotic signaling components (pJNK, p53, and BAX), while concurrently downregulating p62/SQSTM1 expression.
conclusionsOur findings highlight the dual impact of CR on testicular aging, on the one hand the significant attenuation of oxi-inflammation, on the other hand the impairment of germinal epithelial physiology. These results underscore the need for further studies to define optimal timing and duration of dietary interventions aimed at mitigating age-related decline in male reproductive function.
Indexed as
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41511748What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.