Evidence map›Paper›PMID 41511987›Full record

ArticlePloS one2026

Characterization of extended-spectrum cephalosporin-resistant Klebsiella recovered from dairy manure in Southern Ontario, Canada.

Rebecca E V Anderson, Gabhan Chalmers, Andrew Scott, Roger Murray, Michael Mulvey, Edward Topp, Patrick Boerlin, Nicole Ricker

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Rebecca E V AndersonDepartment of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.ORCID https://orcid.org/0009-0002-7515-8649
Gabhan ChalmersDepartment of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Andrew ScottAgriculture and AgriFood Canada, London, Ontario, Canada.
Roger MurrayAgriculture and AgriFood Canada, London, Ontario, Canada.
Michael MulveyNational Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
Edward ToppINRAE, University of Burgundy, Dijon, France.
Patrick BoerlinDepartment of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Nicole RickerDepartment of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.ORCID https://orcid.org/0000-0001-5706-5399

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extended-spectrum cephalosporin (ESC)-resistant Klebsiella pneumoniae are a problem in human patients and have been studied extensively. However, there is a paucity of information regarding ESC-resistant K. pneumoniae from livestock in general, and in Canada in particular. This study characterized ESC-resistant K. pneumoniae recovered from dairy manure in Ontario, Canada, and their ESC-resistance plasmids. ESC-resistant K. pneumoniae (n = 73) and K. quasipneumoniae (n = 11) isolates were screened by PCR for blaCTX-M, blaCMY and blaSHV prior to undergoing antimicrobial susceptibility testing using disk diffusion. Isolates from dairy manure carrying blaCTX-M (n = 74), and additional isolates from turkeys (n = 8) and dogs (n = 2), underwent short-read whole genome sequencing (WGS) and a subset of these (n = 35) had additional long-read sequencing and hybrid assembly for confirmation. Isolates were characterized using multi-locus sequence typing (MLST) and antimicrobial resistance (AMR) gene profiles. Thirty known sequence types (STs) and four novel STs were identified for K. pneumoniae, and two STs among K. quasipneumoniae. The isolates were found at various treatment stages of the manure on one farm but were only sporadically found on any of the other farms investigated. The majority of isolates (86%) were multi-drug resistant. Variants of CTX-M were identified in diverse STs and included blaCTX-M-15 (n = 81), blaCTX-M-1 (n = 2) and blaCTX-M-32 (n = 1). The blaCTX-M-15 gene was located on diverse IncF, IncHI1 or IncY replicons as well as on the chromosome, whereas blaCTX-M-1 was harboured on the epidemic IncI1/ST3 plasmid, and blaCTX-M-32 on an IncN plasmid. Plasmids were characterized based on core gene SNPs, replicon types and AMR genes, and compared to plasmids from Escherichia coli from a parallel study. Transfer of blaCTX-M plasmids between bacterial species by conjugation was also assessed. Conjugation of IncI1 and IncFII plasmids occurred from K. pneumoniae to E. coli strains but not vice versa. Notably, IncY replicons were identified as conjugative plasmids and transfer was demonstrated between E. coli strains as well as from K. pneumoniae to E. coli. No strain overlap was observed between dairy manure isolates and those from turkey and dogs, however we did identify similarities between K. pneumoniae MLST and resistance profiles from publicly available human clinical isolates to our isolates found in dairy manure (ST37 and 405), turkeys (ST45) and dogs (ST711).

Indexed as

CephalosporinsKlebsiellaKlebsiella pneumoniaeManureAnimalsAnti-Bacterial Agentsbeta-LactamasesCattleMicrobial Sensitivity TestsMultilocus Sequence TypingOntarioPlasmidsThird Generation CephalosporinsWhole Genome SequencingAnti-Bacterial Agentsbeta-LactamasesCephalosporinsManureThird Generation Cephalosporins

Identifiers

PMID41511987
PMCPMC12788680

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.