Evidence map›Paper›PMID 41512016›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2026

Assessing cerebral capillary function and stalling using single capillary reporters in ultrasound localization microscopy.

Stephen A Lee, Alexis Leconte, Alice Wu, Joshua Kinugasa, Gerardo Ramos Palacios, Jonathan Porée, Abbas F Sadikot, Andreas Linninger, Jean Provost

Abstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Capillary transit time heterogeneity comes into focus.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  3. Assessing cerebral capillary function and stalling using single capillary reporters in ultrasound localization microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  4. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Stephen A LeeDepartment of Engineering Physics, Polytechnique Montreal, Montreal, QC H3T 1J4, Canada.ORCID 0000-0002-1315-4266
Alexis LeconteDepartment of Engineering Physics, Polytechnique Montreal, Montreal, QC H3T 1J4, Canada.ORCID 0009-0001-2420-9250
Alice WuDepartment of Engineering Physics, Polytechnique Montreal, Montreal, QC H3T 1J4, Canada.ORCID 0009-0001-9914-5191
Joshua KinugasaDepartment of Biomedical Engineering, Chiba University, Chiba 263-8522, Japan.
Gerardo Ramos PalaciosDepartment of Neurology and Neurosurgery, McGill University, Montreal, QC H3A 2B4, Canada.ORCID 0000-0003-0861-6152
Jonathan PoréeDepartment of Engineering Physics, Polytechnique Montreal, Montreal, QC H3T 1J4, Canada.ORCID 0000-0002-4179-9264
Abbas F SadikotDepartment of Neurology and Neurosurgery, McGill University, Montreal, QC H3A 2B4, Canada.
Andreas LinningerDepartment of Biomedical Engineering, University of Illinois, Chicago, IL 60607.ORCID 0000-0002-2694-630X
Jean ProvostDepartment of Engineering Physics, Polytechnique Montreal, Montreal, QC H3T 1J4, Canada.ORCID 0000-0003-2057-2199

Funding

Image-based cerebrovascular network snythesis(iCNS) to model Alzheimer's DiseaseR01AG079894 · NIA · UNIVERSITY OF ILLINOIS AT CHICAGO · PI ANDREAS A LINNINGER · 2023 to 2026
$2.9M
Canada First Research Excellence Fund (CFREF) N/Acanadian foundation for innovation grant 38095Canadian Institutes of Health Research (CIHR) 452530CIHR | Institute of Aging (IA) Postdoctoral FellowshipCONAHCYT N/AFRQ | Nature et technologies (FRQNT) N/AHHS | NIH | National Institute on Aging (NIA) 1R01AG079894Institut de Valorisation des Données (IVADO) N/AInstitut TransMedTech (iTMT) Living Lab Postdoctoral ScholarshipNatural Sciences and Engineering Research Council of Canada (NSERC) RGPIN-2019-04982Natural Sciences and Engineering Research Council of Canada (NSERC) Vanier Banting Postdoctoral FellowshipNew Frontiers in Research Fund NFRFE-2018-01312NIA NIH HHS R01 AG079894Quebec Bio-Imaging Network N/AThe Healthy Brains Healthy Lives N/AThe Insightec N/A
6 · The paper itself

Abstract

While transcranial neuroimaging of individual capillary function holds transformative potential for diagnostics, it has proven difficult to achieve. Superresolution ultrasound, while capable of achieving micron-scale resolution, relies on the accumulation of multiple microbubble events, a method inherently limited by the exceedingly low probability of observing such events within capillaries. We present single capillary reporters (SCaRe), a paradigm-shifting approach that utilizes the complete flow trajectory information extracted from individual microbubbles to directly image single capillaries. This method allows for transcranial reconstruction and functional assessment of deep capillary networks in the entire brain. We employed computational simulations and pathological neuroinflammation models to quantify and validate metrics such as capillary transit-time and capillary stalling. Importantly, we demonstrated SCaRe's ability to resolve immune responses to injury at the single capillary level, markedly broadening research avenues for exploring microvascular dysfunction across diverse neurological conditions.

Indexed as

BrainCapillariesCerebrovascular CirculationMicroscopyNeuroimagingAnimalsComputer SimulationHumansMicrobubblesUltrasonographycapillary stallingmicrovasculatureneuroimagingultrasound localization microscopy

Identifiers

PMID41512016
PMCPMC12799139

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.