Evidence map›Paper›PMID 41514393›Full record

ArticleBMC plant biology2026

Integrated transcriptomic and proteomic analysis reveals inhibitory effects of naringenin application on soybean roots mainly by interfering with auxin and ROS concentrations.

Zeyu Zhang, Bingyan Chen, Yinghe Zhang, Xiaohui Mo, Xing Lu, Tianqi Wang, Cuiyue Liang, Jiang Tian

Abstract read
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In one paragraph

Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Zeyu ZhangRoot Biology Center, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
Bingyan ChenRoot Biology Center, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
Yinghe ZhangRoot Biology Center, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
Xiaohui MoRoot Biology Center, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
Xing LuRoot Biology Center, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
Tianqi WangRoot Biology Center, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
Cuiyue LiangRoot Biology Center, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
Jiang TianRoot Biology Center, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, People's Republic of China. jtian@scau.edu.cn.

Funding

Guangdong Basic and Applied Basic Research Foundation 2024A1515013054Key Areas Research and Development Programs of Guangdong Province 2022B0202060005National Key Research and Development Program of China 2023YFD1901300National Natural Science Foundation of China 32172658, 32172659Open Competition Program of Ten Major Directions of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province 2022SDZG07STI 2030-Major Project 2023ZD04072STIC Gran SGDX20210823103535007
6 · The paper itself

Abstract

backgroundFlavonoids play a crucial role as secondary metabolites in plant growth and development. Naringenin is a central intermediate in flavonoid biosynthesis. It is also a pivotal branching point that gives rise to metabolites such as flavonols, anthocyanins, and isoflavonoids. However, the mechanisms underlying plant root development regulated by flavonoids remain fragmentary, especially in crops.

resultsIn this study, we observed a significant inhibition of soybean (Glycine max) root growth after exposure to 75 μM naringenin. The treated roots showed reduced auxin content and increased reactive oxygen species (ROS) levels in root tips. Through RNA-sequencing and nanoElute high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analyses, we further investigated molecular responses of soybean roots to naringenin treatment. A total of 6,390 differentially expressed genes (DEGs) were identified. Among them, 3,910 genes were up-regulated and 2,480 were down-regulated. These DEGs notably included genes related to ROS metabolism and the auxin signaling pathway. We also detected 806 differential accumulation proteins (DAPs), including 481 up-regulated and 325 down-regulated proteins. The integration of transcriptomic and proteomic data showed a close association between DEGs and DAPs in roots, especially in pathways related to glutathione metabolism.

conclusionTaken together, this comprehensive analysis highlights the intricate and specific responses of soybean roots to naringenin exposure, mainly affecting the levels of auxin and ROS. It provides valuable insights and candidate genes for further investigating root growth regulated by flavonoids.

Indexed as

FlavanonesGlycine maxIndoleacetic AcidsPlant RootsReactive Oxygen SpeciesTranscriptomeGene Expression ProfilingGene Expression Regulation, PlantPlant Growth RegulatorsPlant ProteinsProteomicsFlavanonesIndoleacetic AcidsnaringeninPlant Growth RegulatorsPlant ProteinsReactive Oxygen SpeciesNaringeninProteomicsSoybean rootsTranscriptomics

Identifiers

PMID41514393
PMCPMC12882313

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LicenceCC BY-NC-ND
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.