ArticleBiotechnology for biofuels and bioproducts2026
Bioconversion of lignin and food waste into vanillin and biohydrogen by Escherichia coli MC3: a novel dual-functional bacteria.
Article in Biotechnology for biofuels and bioproducts, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study introduces Escherichia coli MC3, demonstrating unique dual functionality in coupling lignin depolymerization with biohydrogen production from untreated food waste, thereby advancing sustainable biorefineries without energy-intensive pretreatments. MC3 demonstrated a lignin degradation rate of 50.3 ± 3.2% and an Azure B decolorization rate of 34.8 ± 2.1% over a 7-day period, as validated by UV-visible spectrophotometry. High-performance liquid chromatography identified ferulic acid (2.355 mg/L) and vanillin (1.018 mg/L) as primary byproducts of lignin degradation, thereby confirming its ligninolytic potential. Enzymatic assays revealed significant hydrolytic activity, with hydrolysis zones measuring 25.4 ± 1 mm (proteases), 28.1 ± 2 mm (cellulases), 20.5 ± 3 mm (xylanases), and 6.8 ± 1 mm (amylases). Whole-genome sequencing of the E. coli strain MC3 revealed a total of 4798 coding sequences. Among these, key genes identified include carbohydrate-active enzymes (CAZymes: AA2, AA3, CE1), hydrolytic enzymes (GH2, GH13, GH8), multicopper oxidases (CueO, CopA), and biohydrogen-related enzymes (NikA, FbpC). These findings highlight the potential of MC3 for lignin degradation and hydrogen production. In batch fermentation assays, the strain demonstrated a hydrogen yield ranging from 0.292 to 1.004 mol H2 per mol of substrate across various substrates, including xylose, glucose, carboxymethyl cellulose, starch, and food waste, with the highest yield obtained from food waste. The E. coli strain MC3 produced the highest cumulative hydrogen yield of 175 ± 10 mL H₂/g VS from food waste. This study confirms the dual function of E. coli strain MC3 for conversion of lignin into vanillin and biomass into biohydrogen, suggesting the excluding energy-intensive pretreatments, substantial potential for sustainable biorefineries.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.