ArticleAnimals : an open access journal from MDPI2025
LPS-Stressed Bovine Endometrial Cells upon Morulae in a Transwell Model of Embryo-Maternal Talk.
Article in Animals : an open access journal from MDPI, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed, 1 synthesis or guideline pooled it.
- Embryo-derived extracellular vesicles and their potential as biomarkers for embryo quality during IVF: a systematic review.Human reproduction update · 2026Pooled it
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During the preimplantation period, the nutrition of the embryo is dependent on luminal secretions of the uterus, which can be modified by the health status of the animal. The aim of this study was to mimic the paracrine communication between healthy or LPS-stressed epithelial endometrial cells (EECs) and embryos using aa transwell plate. The rate of in vitro embryo production, size, and concentration of extracellular vesicles (EVs), and level of secretion of Galectin-9 (Gal-9) and leukaemia inhibitory factor (LIF) were detected. Embryos were produced with an established protocol of oocyte in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro embryo culture (IVC). On day 55 of IVC, one hour before the transfer of morulae in the basolateral compartment of the transwell, EECs were treated with 10 ng/mL of LPS, and IVC was continued until the eleventh day. Extracellular vesicles (EVs) were obtained from IVC medium by ultracentrifugation. Levels of Gal-9 and LIF were evaluated by ELISA. On day 7, the results did not show statistically different blastocyst rates between EECs+Embryo and EECs+LPS+Embryo (34.94 ± 1.95% and 33.06 ± 3.08%, respectively). On day 11, the rate of hatched blastocysts was 23.03 ± 3.18% in EECs+Embryo, while in EECs+LPS+Embryo, no hatching was observed. Nanosight revealed higher values in EV size and concentration in EECs+LPS+Embryo medium compared to EECs+Embryo (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.