Stage-Specific Alternative Polyadenylation During Human Neural Differentiation Revealed by Integrated Long- and Short-Read Sequencing.

Zheqi Lou, Xianyan Zeng, Tinghui Jiang, Peizhen Du, Jiyao Rao, Xinyan Dai, Haishuang Lin, Yong Zhu

Abstract read

In one paragraph

Article in Biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Zheqi LouCollege of Basic Medicine, Chongqing Medical University, Chongqing 400016, China.

Xianyan ZengCollege of Basic Medicine, Chongqing Medical University, Chongqing 400016, China.

Tinghui JiangCollege of Basic Medicine, Chongqing Medical University, Chongqing 400016, China.

Peizhen DuCollege of Basic Medicine, Chongqing Medical University, Chongqing 400016, China.

Jiyao RaoCollege of Basic Medicine, Chongqing Medical University, Chongqing 400016, China.

Xinyan DaiCollege of Basic Medicine, Chongqing Medical University, Chongqing 400016, China.

Haishuang LinSchool of Biomedical Engineering, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230000, China.ORCID 0000-0002-2601-3278

Yong ZhuCollege of Basic Medicine, Chongqing Medical University, Chongqing 400016, China.ORCID 0000-0002-4645-9883

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human embryonic stem cell (hESC) neural differentiation involves extensive APA; however, reliance on short-read sequencing in prior studies has offered only a limited view of its complexity and dynamic regulation. Here, we integrated Oxford Nanopore (ONT) long-read sequencing with Illumina short-read sequencing to systematically map the APA landscape during early hESC neural differentiation. Our hybrid approach uncovered remarkable transcriptomic complexity, identifying 20,823 novel transcripts and 8241 previously unannotated poly(A) sites (PASs). We characterized distinct dynamic patterns of 3' UTR-APA across differentiation and pinpointed

Indexed as

alternative polyadenylationexon alternative polyadenylationhuman embryonic stem cellsintronic alternative polyadenylationneural differentiationOxford Nanopore Technologiesshort-read sequencing

Identifiers

PMID41514865

DOI10.3390/biology15010024

PMCPMC12784838

What Socratic holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.