Evidence map›Paper›PMID 41519874›Full record

ArticleScientific reports2026

Development and characterization of chicken lung organoids for in vitro modeling of avian influenza virus-host cell interaction.

Hannah F Nicholson, Christopher Zdyrski, Christina M Leyson, Megan P Corbett, Nirmal Kumar, Michael Catucci, Bryan J Melvin, Lisa J Stabler, Seema S Lakdawala, Eugene Douglass and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Hannah F NicholsonPrecision One Health Initiative, College of Veterinary Medicine, University of Georgia, Athens, GA, USA. nicholson.hf@gmail.com.
Christopher ZdyrskiPrecision One Health Initiative, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
Christina M LeysonDepartment of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, USA.
Megan P CorbettPrecision One Health Initiative, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
Nirmal KumarDepartment of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, USA.
Michael CatucciPrecision One Health Initiative, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
Bryan J MelvinPrecision One Health Initiative, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
Lisa J StablerPopulation Health, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
Seema S LakdawalaDepartment of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, USA.
Eugene DouglassPharmaceutical and Biomedical Sciences, Institute of Bioinformatics, University of Georgia, Athens, GA, USA.
Anice C LowenDepartment of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, USA.
Jonathan P MochelPrecision One Health Initiative, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
Karin AllenspachPrecision One Health Initiative, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
Silvia CarnacciniPopulation Health, College of Veterinary Medicine, University of Georgia, Athens, GA, USA. carnacc@iastate.edu.

Funding

JEOL 2100PLUS(CR)S10OD034282 · OD · UNIVERSITY OF GEORGIA · PI SALGUERO, TINA · 2023 to 2023
$1.2M
NIH HHS S10 OD034282
6 · The paper itself

Abstract

High pathogenicity avian influenza viruses pose a growing threat to poultry, livestock, wildlife, and humans as they undergo accelerated expansion of host and geographical ranges. Since 2020, these viruses have driven a panzootic characterized by extensive viral diversification and spillover into species previously considered to be resistant. There is currently a lack of physiologically relevant in vitro models that can be used to screen the rapidly changing viral landscape. To address this need, we describe the first chicken lung organoids derived from adult stem cells of specific pathogen free White Leghorns. We analyze their gene expression with bulk RNA sequencing, confirm their cellular heterogeneity via single-nuclei RNA sequencing, and provide basic morphological characterization using hematoxylin and eosin staining, immunohistochemistry, and transmission electron microscopy. The results indicate that the organoids contained several cell types, including non-ciliated columnar, cuboidal, squamous, and mucin-producing cells, representative of different regions of the avian respiratory system. Furthermore, expression of genes relevant to influenza A virus infection and replication appeared to be conserved across organoid and tissue samples. Infections revealed that chicken lung organoids support robust replication of both low and high pathogenicity avian influenza A viruses, with high pathogenicity strains showing more rapid amplification. Therefore, these organoids have the potential to effectively model viral infection, enabling the investigation of viral pathogenesis and evolutionary potential, virus-host interactions, and antiviral targets.

Indexed as

Host-Pathogen InteractionsInfluenza A virusInfluenza in BirdsLungOrganoidsAnimalsChickensVirus ReplicationAvianChickenInfluenzaLungOrganoidsSingle-nuclei RNA-seqStem cellsVirology

Identifiers

PMID41519874
PMCPMC12800103

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.