Evidence map›Paper›PMID 41524689›Full record

ArticleThe Journal of cell biology2026

Identification of a cytosol-to-nucleus feedback loop that regulates neuronal microtubule nucleation.

Nitish Kumar, Nathaniel Carey, J Ian Hertzler, Annabelle R Bernard, Gibarni Mahata, Gregory O Kothe, Yitao Shen, Melissa M Rolls

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of cell biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Nitish KumarBiochemistry and Molecular Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University , University Park, PA, USA.ORCID 0009-0006-0661-9463
Nathaniel CareyBiochemistry and Molecular Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University , University Park, PA, USA.ORCID 0009-0009-4799-4851
J Ian HertzlerBiochemistry and Molecular Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University , University Park, PA, USA.ORCID 0000-0001-8025-8367
Annabelle R BernardBiochemistry and Molecular Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University , University Park, PA, USA.ORCID 0000-0002-3339-1584
Gibarni MahataBiochemistry and Molecular Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University , University Park, PA, USA.ORCID 0000-0003-2867-4130
Gregory O KotheBiochemistry and Molecular Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University , University Park, PA, USA.ORCID 0009-0004-8430-3988
Yitao ShenBiochemistry and Molecular Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University , University Park, PA, USA.ORCID 0000-0001-7956-8237
Melissa M RollsBiochemistry and Molecular Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University , University Park, PA, USA.ORCID 0000-0002-5021-4360

Funding

Resource Component: Acquisition, maintenance and distribution of Drosophila stocksP40OD018537 · OD · TRUSTEES OF INDIANA UNIVERSITY · PI Annette L. Parks · 2014 to 2026
$13.5M
Using Drosophila Neurons to Identify Mechanisms that Control Microtubule PolarityR01GM085115 · NIGMS · PENNSYLVANIA STATE UNIVERSITY, THE · PI Melissa Rolls · 2010 to 2026
$5.1M
Function of kinetochore proteins in post-mitotic neuronsR21NS117396 · NINDS · PENNSYLVANIA STATE UNIVERSITY, THE · PI ROLLS, MELISSA · 2020 to 2020
$434k
NIGMS NIH HHS R01 GM085115NIGMS NIH HHS R01GM085115NIH HHS P40 OD018537NINDS NIH HHS R21 NS117396NINDS NIH HHS R21NS117396
6 · The paper itself

Abstract

Maintenance of the microtubule cytoskeleton is critical for long-term neuronal function. From a deficiency screen in Drosophila larvae, we identified the spindle matrix (SM) protein Skeletor as a regulator of microtubule dynamics in dendrites. With other SM components, Chromator and Megator, Skeletor controls the expression of γTubulin, the core microtubule-nucleating protein, in neurons. Surprisingly, the SM proteins localize to different places in neurons: Megator to nuclear pores, Chromator to the nucleus, and Skeletor to the cytoplasm and nucleus. To test whether they function to sense and regulate microtubule dynamics across cellular compartments, we increased microtubule dynamics and monitored nuclear Chromator. When microtubule dynamics was increased, Chromator levels in the nucleus were reduced in a manner depending on Skeletor and Megator. Moreover, the overexpression of Chromator was sufficient to increase γTubulin and microtubule dynamics. We propose that Skeletor and Megator communicate changes in the microtubule state to Chromator in the nucleus as part of a negative feedback loop that regulates microtubule nucleation.

Indexed as

Cell NucleusCytosolDrosophila melanogasterDrosophila ProteinsMicrotubulesNeuronsAnimalsFeedback, PhysiologicalMicrotubule-Associated ProteinsTubulinDrosophila ProteinsMicrotubule-Associated ProteinsTubulin

Identifiers

PMID41524689
PMCPMC12828650

What Socratic holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.