Evidence map›Paper›PMID 41524754›Full record

ArticleArchives of microbiology2026

A tRNA modification-based regulatory strategy for Lincomycin biosynthesis in Streptomyces lincolnensis.

Yue Mao, Jiang Ye, Ruida Wang, Haizhen Wu, Huizhan Zhang

Abstract read
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Article in Archives of microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yue MaoState Key Laboratory of Bioreactor Engineering, Department of Applied Biology, Bioengineering Experiment Teaching Demonstration Center, School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.
Jiang YeState Key Laboratory of Bioreactor Engineering, Department of Applied Biology, Bioengineering Experiment Teaching Demonstration Center, School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.
Ruida WangState Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in Tarim Basin, College of Life Science and Technology, Tarim University, Alar, 843300, China. wrd@taru.edu.cm.
Haizhen WuState Key Laboratory of Bioreactor Engineering, Department of Applied Biology, Bioengineering Experiment Teaching Demonstration Center, School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China. wuhzh@ecust.edu.cn.
Huizhan ZhangState Key Laboratory of Bioreactor Engineering, Department of Applied Biology, Bioengineering Experiment Teaching Demonstration Center, School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.

Funding

National Key Research and Development Program of China 2021YFC2100600National Natural Science Foundation of China 42406126President's Fund of Tarim University TDZKBS202536
6 · The paper itself

Abstract

Lincomycin is a clinically important lincosamide antibiotic. Its biosynthetic efficiency is limited by the presence of the rare UUA codon in the key positive regulators LmbU and AdpA. In this study, we developed a universal metabolic optimization strategy based on tRNA modification engineering. Co-overexpression of the UUA-decoding tRNA gene bldA and its post-transcriptional modification enzyme gene miaA markedly increased lincomycin production. Further optimization was achieved by expressing miaA under the phase-dependent promoter PlmbU. Mechanistic analysis showed that this strategy enhances the decoding efficiency of the UUA codon, thereby improving translation of LmbU and AdpA. Because bldA and miaA are highly conserved among species of Streptomyces, this approach may be broadly applicable to other rare-codon-dependent secondary metabolites. Our work provides a new and rational strategy for microbial secondary metabolic engineering through precise control of tRNA modification.

Indexed as

Anti-Bacterial AgentsLincomycinRNA, TransferStreptomycesBacterial ProteinsCodonGene Expression Regulation, BacterialMetabolic EngineeringAnti-Bacterial AgentsBacterial ProteinsCodonLincomycinRNA, TransferBldALincomycinMetabolic engineeringStreptomyces lincolnensistRNA modification

Identifiers

PMID41524754

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.