Evidence map›Paper›PMID 41524965›Full record

ArticlePurinergic signalling2026

Capsaicin suppresses LPS-induced inflammatory responses via NLRP3/CASP-1/IL-1β axis and purinergic pathways in BV-2 microglial cells.

Bianca Vedoin Copês Rambo, Milagros Fanny Vera Castro, Mairin Schott, Robson Lourenço da Silva Santos, Charles Elias Assmann, Marcylene Vieira da Silveira, Pâmela de Almeida Milioni, Adriel Antonio Schirmann, Vitor Bastianello Mostardeiro, Nathieli Bianchin Bottari and 2 more

Abstract read
In one paragraph

Article in Purinergic signalling, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Bianca Vedoin Copês RamboPostgraduate Program in Biological Sciences: Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil. bianca.rambo@acad.ufsm.br.ORCID 0009-0005-9770-3021
Milagros Fanny Vera CastroPostgraduate Program in Biological Sciences: Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.ORCID 0000-0001-9274-2877
Mairin SchottPostgraduate Program in Biological Sciences: Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.ORCID 0000-0002-4248-5240
Robson Lourenço da Silva SantosPostgraduate Program in Biological Sciences: Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.ORCID 0000-0002-5243-6581
Charles Elias AssmannPostgraduate Program in Biological Sciences: Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.ORCID 0000-0002-3524-3446
Marcylene Vieira da SilveiraPostgraduate Program in Biological Sciences: Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.ORCID 0000-0001-7825-3903
Pâmela de Almeida MilioniPostgraduate Program in Biological Sciences: Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.ORCID 0009-0002-2604-4418
Adriel Antonio SchirmannPostgraduate Program in Biological Sciences: Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.ORCID 0009-0003-8940-9565
Vitor Bastianello MostardeiroPostgraduate Program in Biological Sciences: Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.ORCID 0000-0003-4320-0400
Nathieli Bianchin BottariPostgraduate Program in Microbiology and Parasitology, Department of Microbiology and Parasitology, Federal University of Pelotas (UFPEL), Pelotas, RS, Brazil.ORCID 0000-0003-0294-9650
Maria Rosa Chitolina SchetingerPostgraduate Program in Biological Sciences: Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.ORCID 0000-0002-5240-8935
Vera Maria Melchiors MorschPostgraduate Program in Biological Sciences: Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.ORCID 0000-0002-5381-4556

Funding

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 88887.821555/2023-00
6 · The paper itself

Abstract

Microglial activation drives neuroinflammation, a key factor in many neurological diseases. The purinergic system is a major regulator of inflammatory responses and represents a promising target for controlling neuroinflammation. Capsaicin, a bioactive compound found in chili peppers, exhibits significant anti-inflammatory and antioxidant properties. This study aimed to investigate the modulatory effects of capsaicin on microglial activation and purinergic system regulation. For this, BV-2 microglial cells were exposed to lipopolysaccharide (1 μg/mL) and treated with capsaicin (25 and 50 μM) for 24 hours. Cell viability was assessed by MTT and trypan blue assays. Cell cycle and apoptosis were evaluated by flow cytometry. Nitric oxide, reactive species and malondialdehyde levels were evaluated as markers of oxidative stress. Activities of NTPDase, 5'-nucleotidase (5'-NT), and adenosine deaminase (ADA) were evaluated. Gene expression of inflammatory mediators and purinergic receptors were analyzed by qRT-PCR, and molecular docking analyses were performed. As a result, capsaicin decreased the expression of pro-inflammatory mediators (NLRP3, CASP-1, IL-1β, IL-6, and TNF-α), increased IL-10 expression, and attenuated oxidative stress. It reduced NTPDase, 5'-NT, and ADA activities, downregulated P2X7 and A2A receptor expression, and upregulated A1 receptor expression. Molecular docking revealed that capsaicin has a high affinity for the A1 and A2A receptors, as well as for ADA. Collectively, these findings suggest that capsaicin exerts neuroprotective effect by suppressing pro-inflammatory signaling, enhancing anti-inflammatory responses, reducing oxidative stress, and modulating key components of the purinergic system, including ectoenzyme activities and P2X7, A1, and A2A receptor expression.

Indexed as

CapsaicinInflammationInterleukin-1betaMicrogliaNLR Family, Pyrin Domain-Containing 3 ProteinAnimalsCaspase 1Cell LineCell SurvivalLipopolysaccharidesMiceOxidative StressReceptors, PurinergicSignal TransductionCapsaicinCaspase 1Interleukin-1betaLipopolysaccharidesNLR Family, Pyrin Domain-Containing 3 ProteinNlrp3 protein, mouseReceptors, PurinergicCapsaicinLipopolysaccharideMicrogliaNeuroinflammationPurinergic System

Identifiers

PMID41524965
PMCPMC12796030

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.