ArticleJournal of mass spectrometry : JMS2026
Demonstrating Voxel-by-Voxel (V × V) Single-Point Calibration in Whole-Body Zebrafish by IR-MALDESI Quantitative MSI.
Article in Journal of mass spectrometry : JMS, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Demonstrating Voxel-by-Voxel (V × V) Single-Point Calibration in Whole-Body Zebrafish by IR-MALDESI Quantitative MSI.Journal of mass spectrometry : JMS · 2026Article
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Authors and funding
3 authors.
Funding
Abstract
This work describes a quantitative mass spectrometry imaging (qMSI) method comparison for the absolute quantification of arachidonic acid (AA) in whole-body zebrafish using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI). Whole-body zebrafish are structurally heterogeneous samples that are complex to analyze by many qMSI methods largely due to practical sample preparation limitations. However, the multi-organ quantification is valuable in zebrafish, especially for lipid-related investigations. In the standard workflow, the ion abundance of AA was normalized to a structural analogue that was sprayed on the slide before mounting the tissue. A series of calibration spots of stable isotope label (SIL) deuterated AA were spotted onto tissue to construct a calibration curve and subsequently calculate the concentration of endogenous AA in the tissue sections. The calculated values of AA using this method provided values significantly lower than the literature. In the subsequent workflow, the structural analogue was considered in a voxel-by-voxel (V × V) calculation of the concentration of AA in the tissues resulting in an AA concentration similar to the literature within whole-body zebrafish. The V × V method proved simpler in sample preparation, and more accurately quantified AA. This implies the potential utility of single-point V × V calibration for the qMSI of highly heterogeneous tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.