Evidence mapPaperPMID 41527019Full record

ArticleBMC microbiology2026

Gut microbiota mediates the beneficial effects of exercise on autism-like behaviors.

Jiugen Zhong, Baoyuan Zhu, Zhi Zou, Yinhua Li, Yanqing Feng, Kai Wu, Xiaohui Hou

Abstract read
In one paragraph

Article in BMC microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 1 pooled it
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Dissecting the Ecological Structure of Health and Disease in the Global Gut Microbiome.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  3. Article
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  5. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jiugen Zhong *Guangdong Provincial Key Laboratory of Physical Activity and Health Promotion, Guangzhou, Guangdong Province, 500510, China.
Baoyuan Zhu *School of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou International Campus, Guangzhou, Guangdong Province, 511442, China.
Zhi ZouGuangdong Provincial Key Laboratory of Physical Activity and Health Promotion, Guangzhou, Guangdong Province, 500510, China.
Yinhua LiGuangdong Provincial Key Laboratory of Physical Activity and Health Promotion, Guangzhou, Guangdong Province, 500510, China.
Yanqing FengGuangdong Provincial Key Laboratory of Physical Activity and Health Promotion, Guangzhou, Guangdong Province, 500510, China.
Kai WuSchool of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou International Campus, Guangzhou, Guangdong Province, 511442, China. kaiwu@scut.edu.cn.
Xiaohui HouGuangdong Provincial Key Laboratory of Physical Activity and Health Promotion, Guangzhou, Guangdong Province, 500510, China. houxh@gzsport.edu.cn.

Funding

the Guangzhou Municipal Science and Technology Bureau, Basic Research Program no. 2025A04J4356the Key-Area Research and Development Program of Guangdong Province no. 2023B0303020001the Provincial Significant Scientific Research Projects for General Universities in Guangdong Province no. 2021ZDJS021
6 · The paper itself

Abstract

backgroundThe gut-brain axis plays a critical role in autism spectrum disorder (ASD), but the mechanisms through which exercise modulates gut microbiota, short-chain fatty acids (SCFAs), and central neurotransmitters to ameliorate ASD-like behaviors remain unclear. This study aimed to investigate the effects of exercise on ASD-like behaviors, gut microbiota, and metabolism in a valproic acid (VPA)-induced ASD rat model and to validate these findings via fecal microbiota transplantation (FMT).

methodsASD rat models were established through prenatal exposure to VPA and divided into four groups: exercise (E_ASD), non-exercise (ASD), FMT, and sham FMT (sFMT). The E_ASD group underwent 6 weeks of voluntary wheel running, while the FMT group received fecal microbiota from the E_ASD group for 4 weeks. Behavioral assessments were conducted to evaluate cognitive and social functions. Fecal microbiota composition was analyzed via 16S rRNA sequencing, while SCFAs and neurotransmitters were measured using gas and liquid chromatography-mass spectrometry.

resultsSix weeks of voluntary exercise significantly alleviated ASD-like behaviors, particularly improving social interactions. Exercise also altered gut microbiota composition, increasing Limosilactobacillus and Lactobacillus while decreasing Allobaculum. Additionally, SCFAs and neurotransmitter levels in the prefrontal cortex were modulated. Notably, FMT from the exercise group replicated these behavioral and metabolic improvements in ASD rats. Exercise improves ASD-like behaviors by modulating gut microbiota, SCFAs, and neurotransmitter levels, and FMT offers further validation of these effects.

conclusionThese findings highlight exercise and FMT as promising strategies for alleviating ASD-related symptoms through gut-brain axis modulation.

Indexed as

Autism Spectrum DisorderAutistic DisorderGastrointestinal MicrobiomePhysical Conditioning, AnimalAnimalsBacteriaBehavior, AnimalDisease Models, AnimalFatty Acids, VolatileFecal Microbiota TransplantationFecesFemaleMaleNeurotransmitter AgentsPregnancyRatsFatty Acids, VolatileNeurotransmitter AgentsRNA, Ribosomal, 16SValproic AcidAutism spectrum disorderExercise interventionFecal microbiota transplantationGut microbiotaNeurotransmittersShort-chain fatty acids

Identifiers

PMID41527019
PMCPMC12927222

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.