Evidence map›Paper›PMID 41527932›Full record

ArticleGut microbes2026

A novel Mediterranean diet-inspired supplement reduces hippocampal amyloid deposits and microglial activation through the modulation of the microbiota gut-brain axis in 5xFAD mice.

Emily Connell, Gwénaëlle Le Gall, Simon McArthur, Leonie Lang, Bernadette Breeze, Marrium Liaquat, Matthew G Pontifex, Saber Sami, Line Pourtau, David Gaudout and 2 more

Abstract read
In one paragraph

Article in Gut microbes, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Emily ConnellNorwich Medical School, Faculty of Medicine and Health Sciences, University of East Anglia, Norwich, United Kingdom.
Gwénaëlle Le GallNorwich Medical School, Faculty of Medicine and Health Sciences, University of East Anglia, Norwich, United Kingdom.ORCID 0000-0002-1379-2196
Simon McArthurInstitute of Dentistry, Faculty of Medicine & Dentistry, Queen Mary University of London, Blizard Institute, London, United Kingdom.ORCID 0000-0001-8521-1808
Leonie LangNorwich Medical School, Faculty of Medicine and Health Sciences, University of East Anglia, Norwich, United Kingdom.ORCID 0009-0008-6725-663X
Bernadette BreezeNorwich Medical School, Faculty of Medicine and Health Sciences, University of East Anglia, Norwich, United Kingdom.
Marrium LiaquatNorwich Medical School, Faculty of Medicine and Health Sciences, University of East Anglia, Norwich, United Kingdom.ORCID 0000-0001-7290-2554
Matthew G PontifexNorwich Medical School, Faculty of Medicine and Health Sciences, University of East Anglia, Norwich, United Kingdom.ORCID 0000-0003-2174-2313
Saber SamiNorwich Medical School, Faculty of Medicine and Health Sciences, University of East Anglia, Norwich, United Kingdom.ORCID 0000-0001-5345-0388
Line PourtauActiv'Inside, Beychac et Caillau, France.ORCID 0000-0003-3315-2222
David GaudoutActiv'Inside, Beychac et Caillau, France.ORCID 0000-0002-3255-8274
Michael MüllerNorwich Medical School, Faculty of Medicine and Health Sciences, University of East Anglia, Norwich, United Kingdom.ORCID 0000-0002-5930-9905
David VauzourNorwich Medical School, Faculty of Medicine and Health Sciences, University of East Anglia, Norwich, United Kingdom.ORCID 0000-0001-5952-8756

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAlzheimer's disease (AD) is projected to increase in prevalence, heightening the need for strategies to alleviate its neuropathological burden. The bioactive constituents of a Mediterranean-style diet are well-recognised for their neuroprotective properties. Due to their capacity to alter the gut microbiome composition, these benefits may involve modulation of the microbiota-gut-brain axis. In this study, we investigated whether a novel supplement enriched with key Mediterranean diet-derived bioactives (Neurosyn240) could reduce amyloid deposition and microglial activation in 5xFAD mice, a transgenic model of AD, through microbiota-mediated mechanisms.

methodsMale and female 5xFAD transgenic mice (

resultsNeurosyn240 significantly shifted the gut microbiome composition, which was associated with increased circulatory serotonin levels and decreased kynurenine and bile acids (TCA, HDCA, TDCA, CDCA and LCA) concentrations. In the brain, Neurosyn240 consumption led to a significant reduction in hippocampal amyloid deposits and Iba-1 positive microglia (p<0.05), which were associated with decreased LCA and increased serotonin, respectively. Hippocampal RNA sequencing further highlighted the upregulation of genes involved in promoting amyloid beta clearance mechanisms.

conclusionsTogether, these findings highlight novel neuroprotective effects of Neurosyn240 in modulating metabolite-mediated pathways of the microbiota-gut-brain axis, accentuating its therapeutic potential against AD progression.

Indexed as

Alzheimer DiseaseDietary SupplementsDiet, MediterraneanGastrointestinal MicrobiomeHippocampusMicrogliaAmyloid beta-PeptidesAnimalsBrainDisease Models, AnimalFemaleMaleMiceMice, TransgenicAmyloid beta-Peptidesamyloid depositsbraingut microbiomehippocampusMediterranean dietmicrobial-derived metabolitesmicroglia

Identifiers

PMID41527932
PMCPMC12802989

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.