Evidence map›Paper›PMID 41528388›Full record

ArticleNeurochemical research2026

Ginsenoside Rb1 Maintains Tunneling Nanotubes between Astrocytes and Neurons To Protect Mice from Cerebral ischemia/reperfusion Injury.

Wei Chen, Jiugeng Feng, Pandi Chen, Hongcai Wang, Zengpan Li, Jian Yan, Gengfan Ye, Guanhua Zhang, Yaxin Qin

Abstract read
PubMed Publisher
In one paragraph

Article in Neurochemical research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Endothelial energy failure as a therapeutic target in elderly strokes.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Wei Chen *Department of Neurosurgery, The Affiliated Lihuili Hospital of Ningbo University, Ningbo, 315040, Zhejiang, China.
Jiugeng Feng *Department of Neurosurgery, the First Affiliated Hospital of Nanchang University, Nanchang, 330008, Jiangxi, China.
Pandi ChenDepartment of Neurosurgery, The Affiliated Lihuili Hospital of Ningbo University, Ningbo, 315040, Zhejiang, China.
Hongcai WangDepartment of Neurosurgery, The Affiliated Lihuili Hospital of Ningbo University, Ningbo, 315040, Zhejiang, China.
Zengpan LiEmergency and Trauma Center, The Affiliated Lihuili Hospital of Ningbo University, Ningbo, 315040, Zhejiang, China.
Jian YanDepartment of Neurosurgery, the First Affiliated Hospital of Nanchang University, Nanchang, 330008, Jiangxi, China.
Gengfan YeDepartment of Neurosurgery, The Affiliated Lihuili Hospital of Ningbo University, Ningbo, 315040, Zhejiang, China. lhlyegengfan@nbu.edu.cn.
Guanhua ZhangDepartment of Cerebrovascular Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510000, China. zhanggh53@mail.sysu.edu.cn.
Yaxin QinDepartment of Geriatrics, Yixing Traditional Chinese Medicine Hospital, No. 128, Yangquan East Road, Yicheng Sub-district, Yixing, 214200, Jiangsu, China. 15961588564@163.com.

Funding

Jiangxi Provincial and Ministerial level Fund 20212BAB216025the National Natural Science of Foundation 82571565，82171366, 81960236Zhejiang Province Clinical Research Project of Traditional Chinese Medicine 2025-ZL-521Zhejiang Province Natural Science Foundation Exploration Project LMS25H09009Zhejiang Provincial Medical Science and Technology Project 2025-KY-207
6 · The paper itself

Abstract

Ginsenoside Rb1 (GRb1), the major bioactive component of ginseng, exhibits multiple therapeutic effects. However, its neuroprotective role in cerebral ischemia/reperfusion (I/R) injury remains unclear. The neuroprotective effects of GRb1 were investigated using a mouse middle cerebral artery occlusion (MCAO) model and in vitro oxygen-glucose deprivation/reoxygenation (OGD/R) models. GRb1 was administered intraperitoneally to MCAO mice, and the effects on neurological function, brain edema, blood-brain barrier (BBB) integrity, and extracellular matrix (ECM) remodeling were evaluated. In the OGD/R model, tunneling nanotubes (TnTs) formation, oxidative stress, and mitochondrial integrity were evaluated in a co-culture of astrocytes and neurons. GRb1 markedly enhanced neurological function, alleviated brain edema, and maintained BBB integrity in MCAO mice. It also inhibited the expression of matrix metalloproteinases and Granzyme B while increasing Serpina3n, indicating protection of ECM integrity. In OGD/R-treated neurons, GRb1 reduced oxidative stress, restored superoxide dismutase activity, and preserved ATP and mitochondrial DNA. Importantly, GRb1 significantly enhanced TnTs formation, and inhibition of TnTs with cytochalasin B markedly reversed these protective effects, supporting a TnT-dependent mechanism. GRb1 effectively protected against I/R-induced neuronal injury through TnTs-dependent mechanisms, modulating oxidative stress, ECM remodeling, and mitochondrial integrity.

Indexed as

AstrocytesBrain IschemiaGinsenosidesNanotubesNeuronsNeuroprotective AgentsReperfusion InjuryAnimalsBlood-Brain BarrierCell Membrane StructuresCoculture TechniquesInfarction, Middle Cerebral ArteryMaleMiceMice, Inbred C57BLOxidative Stressginsenoside Rb1GinsenosidesNeuroprotective AgentsTunneling NanotubesCerebral ischemia/reperfusion injuryExtracellular matrixGinsenoside Rb1Serpina3nTunneling nanotubes

Identifiers

PMID41528388

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.