Evidence mapPaperPMID 41533591Full record

ArticleNucleic acids research2026

Derepression of a single microRNA target causes female infertility in mice.

Joanna Stefano, Lara E Elcavage, Sue-Jean Hong, David P Bartel, Benjamin Kleaveland

Abstract read
In one paragraph

Article in Nucleic acids research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Joanna StefanoHoward Hughes Medical Institute, Cambridge, MA 02142, United States.
Lara E ElcavageHoward Hughes Medical Institute, Cambridge, MA 02142, United States.ORCID 0000-0003-1457-2109
Sue-Jean HongHoward Hughes Medical Institute, Cambridge, MA 02142, United States.
David P BartelHoward Hughes Medical Institute, Cambridge, MA 02142, United States.ORCID 0000-0002-3872-2856
Benjamin KleavelandDepartment of Pathology and Lab Medicine, Weill Cornell Medicine, New York, NY 10065, United States.ORCID 0000-0002-0333-3586

Funding

Medical Scientist Training ProgramT32GM144273 · NIGMS · HARVARD MEDICAL SCHOOL · 2022 to 2025
$5.6M
MicroRNA genes and their functionsR01GM067031 · WHITEHEAD INSTITUTE FOR BIOMEDICAL RES · 2002 to 2005
$1.6M
Pre-doctoral Training in Fundamental Approaches to Biochemistry and Cell and Molecular BiologyT32GM136540 · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · 2025 to 2025
$1.6M
Post-transcriptional gene regulationR35GM118135 · WHITEHEAD INSTITUTE FOR BIOMEDICAL RES · 2025 to 2025
$734k
Destruction of noncoding RNAs - R35 Diversity SupplementR35GM147463 · WEILL MEDICAL COLL OF CORNELL UNIV · 2025 to 2025
$573k
Characterization of the role of ZSWIM8 and target-directed microRNA degradation in hematopoiesisF30HL175923 · HARVARD MEDICAL SCHOOL · 2025 to 2025
$55k
Howard Hughes Medical InstituteNHLBI NIH HHS F30 HL175923NIGMS NIH HHS F30HL175923NIGMS NIH HHS R01 GM067031NIGMS NIH HHS R35 GM118135NIGMS NIH HHS R35 GM147463NIGMS NIH HHS T32 GM136540NIGMS NIH HHS T32 GM144273NIH HHS R01GM067031NIH HHS R35GM118135NIH HHS R35GM147463NIH HHS T32GM136540NIH HHS T32GM144273
6 · The paper itself

Abstract

The miR-200a and miR-200b families control mouse ovulation and are essential for female fertility. The ZEB1 transcription factor is a conserved target of both families and has been implicated as a key player in female fertility at multiple levels. Using gene-edited mice that express a miR-200a/b-resistant form of Zeb1, we found that derepression of Zeb1 in the female pituitary caused decreased production of luteinizing hormone and anovulatory infertility. These phenotypes were accompanied by widespread changes in pituitary gene expression characterized by decreased levels of ZEB1 targets, which include the miR-200a/b microRNAs (miRNAs), as expected from the miR-200a/b-ZEB1 double-negative feedback loop. Also observed were increased levels of mesenchymal genes, neuronal genes, and miR-200a/b targets. These results show that a double-negative feedback loop centered on the miRNA regulation of a single transcription factor can significantly influence the expression of thousands of genes and have dramatic phenotypic consequences.

Indexed as

Infertility, FemaleMicroRNAsZinc Finger E-box-Binding Homeobox 1AnimalsFemaleGene Expression RegulationLuteinizing HormoneMicePituitary GlandLuteinizing HormoneMicroRNAsMirn200 microRNA, mouseZEB1 protein, mouseZinc Finger E-box-Binding Homeobox 1

Identifiers

PMID41533591
PMCPMC12802903

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.