Evidence mapPaperPMID 41535398Full record

ArticleScientific reports2026

Genetic characterization and whole-genome sequencing-based genetic analysis of influenza B in Shandong Province during 2015-2024.

Julong Wu, Yujie He, Lin Sun, Shu Zhang, Qing Duan, Jian Lv, Shaoxia Song, Jing Liu, Xuedong Zheng, Zhong Li and 3 more

Abstract read
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Julong WuShandong Provincial Center for Disease Prevention and Control, Shandong Province Key Laboratory of Intelligent Monitoring, Early Warning, and Prevention of Infectious Diseases (Preparatory), Jinan, China.
Yujie HeShandong Provincial Center for Disease Prevention and Control, Shandong Province Key Laboratory of Intelligent Monitoring, Early Warning, and Prevention of Infectious Diseases (Preparatory), Jinan, China.
Lin SunShandong Provincial Center for Disease Prevention and Control, Shandong Province Key Laboratory of Intelligent Monitoring, Early Warning, and Prevention of Infectious Diseases (Preparatory), Jinan, China.
Shu ZhangShandong Provincial Center for Disease Prevention and Control, Shandong Province Key Laboratory of Intelligent Monitoring, Early Warning, and Prevention of Infectious Diseases (Preparatory), Jinan, China.
Qing DuanShandong Provincial Center for Disease Prevention and Control, Shandong Province Key Laboratory of Intelligent Monitoring, Early Warning, and Prevention of Infectious Diseases (Preparatory), Jinan, China.
Jian LvShandong Provincial Center for Disease Prevention and Control, Shandong Province Key Laboratory of Intelligent Monitoring, Early Warning, and Prevention of Infectious Diseases (Preparatory), Jinan, China.
Shaoxia SongShandong Provincial Center for Disease Prevention and Control, Shandong Province Key Laboratory of Intelligent Monitoring, Early Warning, and Prevention of Infectious Diseases (Preparatory), Jinan, China.
Jing LiuShandong Provincial Center for Disease Prevention and Control, Shandong Province Key Laboratory of Intelligent Monitoring, Early Warning, and Prevention of Infectious Diseases (Preparatory), Jinan, China.
Xuedong ZhengShandong Provincial Center for Disease Prevention and Control, Shandong Province Key Laboratory of Intelligent Monitoring, Early Warning, and Prevention of Infectious Diseases (Preparatory), Jinan, China.
Zhong LiShandong Provincial Center for Disease Prevention and Control, Shandong Province Key Laboratory of Intelligent Monitoring, Early Warning, and Prevention of Infectious Diseases (Preparatory), Jinan, China.
Xianjun WangShandong Provincial Center for Disease Prevention and Control, Shandong Province Key Laboratory of Intelligent Monitoring, Early Warning, and Prevention of Infectious Diseases (Preparatory), Jinan, China.
Zengqiang KouShandong Provincial Center for Disease Prevention and Control, Shandong Province Key Laboratory of Intelligent Monitoring, Early Warning, and Prevention of Infectious Diseases (Preparatory), Jinan, China.
Ti LiuShandong Provincial Center for Disease Prevention and Control, Shandong Province Key Laboratory of Intelligent Monitoring, Early Warning, and Prevention of Infectious Diseases (Preparatory), Jinan, China. liuti1204@126.com.

Funding

Natural Science Foundation of Shandong Province ZR2021MH372
6 · The paper itself

Abstract

Influenza B virus (IBV) remains a significant concern due to its role in seasonal epidemics, frequently leading to localized outbreaks characterized by high morbidity and mortality rates. This study analyzed 109 IBV strains isolated in Shandong Province using phylogenetic and amino acid variation analyses. These sequences were aligned with vaccine strains and representative isolates obtained from the GISAID influenza database. Phylogenetic trees were constructed using the maximum likelihood (ML) method in MEGA software, while amino acid substitutions were visualized using MegAlign software, and potential N-glycosylation sites were predicted using NetNGlyc 1.0. Compared with contemporary vaccine strains, the 109 IBV strains isolated in Shandong Province exhibited genetic homology ranging from 96.2 to 100.0%, with a trend of increasing evolutionary divergence over time. Annual amino acid substitutions in the HA gene were observed, particularly within critical antigenic epitopes, including the 120-loop, 150-loop, 160-loop, and 190-helix, as well as in receptor-binding domains. A total of 30 putative antigenic variants were identified. Notably, one strain carried the H273Y mutation in the NA gene, which is associated with resistance to neuraminidase (NA) inhibitors. Additional mutations were detected in genes encoding NP, PA, NS1, PB2, and PB1, suggesting their potential involvement in viral replication and immune evasion. Furthermore, one B/Victoria strain was identified with an NP gene derived from the B/Yamagata lineage, indicating possible reassortment between lineages. The genomic evolution of Shandong's IBV strains between 2015 and 2024 was driven by both antigenic drift and genetic reassortment. The suboptimal antigenic match between circulating strains and vaccine components, along with the emergence of drug-resistant variants, underscores the urgent need for continuous vaccine updates and strategic selection of antiviral drugs. Sustained surveillance of IBV epidemiology and genetic evolution remains essential for providing early warnings of potential pandemic threats.

Indexed as

Genome, ViralInfluenza B virusInfluenza, HumanAmino Acid SubstitutionAnimalsChinaEvolution, MolecularHumansMutationNeuraminidasePhylogenyWhole Genome SequencingNeuraminidaseEvolutionary variationInfluenza BInfluenza-like casesWhole genome sequencing

Identifiers

PMID41535398
PMCPMC12880975

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.