Evidence map›Paper›PMID 41537644›Full record

ArticleSmall (Weinheim an der Bergstrasse, Germany)2026

Volumetric Single-Molecule Tracking Inside Subcellular Structures.

Sam Daly, Joseph E Chambers, Caroline Jones, Bin Fu, James D Manton, Joseph S Beckwith, Stefan J Marciniak, David C Gershlick, Steven F Lee

Abstract read
In one paragraph

Article in Small (Weinheim an der Bergstrasse, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Volumetric Single-Molecule Tracking Inside Subcellular Structures.Small (Weinheim an der Bergstrasse, Germany) · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Sam DalyYusuf Hamied Department of Chemistry, Lensfield Road, University of Cambridge, Cambridge, UK.ORCID https://orcid.org/0000-0002-8559-3161
Joseph E ChambersCambridge Institute for Medical Research, University of Cambridge, Cambridge, UK.ORCID https://orcid.org/0000-0003-4675-0053
Caroline JonesYusuf Hamied Department of Chemistry, Lensfield Road, University of Cambridge, Cambridge, UK.ORCID https://orcid.org/0000-0002-3462-1302
Bin FuYusuf Hamied Department of Chemistry, Lensfield Road, University of Cambridge, Cambridge, UK.ORCID https://orcid.org/0000-0002-8816-2906
James D MantonMRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, UK.ORCID https://orcid.org/0000-0001-9260-3156
Joseph S BeckwithYusuf Hamied Department of Chemistry, Lensfield Road, University of Cambridge, Cambridge, UK.ORCID https://orcid.org/0000-0003-4726-230X
Stefan J MarciniakCambridge Institute for Medical Research, University of Cambridge, Cambridge, UK.ORCID https://orcid.org/0000-0001-8472-7183
David C GershlickCambridge Institute for Medical Research, University of Cambridge, Cambridge, UK.ORCID https://orcid.org/0000-0002-0602-210X
Steven F LeeYusuf Hamied Department of Chemistry, Lensfield Road, University of Cambridge, Cambridge, UK.ORCID https://orcid.org/0000-0003-4492-5139

Funding

Alpha-1 Foundation pC ID: 830153Biotechnology and Biological Sciences Research Council BB/X511092/1Biotechnology and Biological Sciences Research Council UKRI715Medical Research Council MR/V028669/1Medical Research Council MR/Y011813/1Royal Society RGF/EA/181021
6 · The paper itself

Abstract

The non-covalent interactions that underpin major cellular functions depend on molecular motion within 3D environments. Large depth-of-field single-molecule localization microscopy (3D-SMLM) methods facilitate these measurements, but their increased optical complexity and bespoke post-processing pipelines often sacrifice important cellular context. Here, we combine single-molecule light-field microscopy (SMLFM) with widefield Fourier light-field microscopy for correlative volumetric organelle imaging. The instantaneous acquisition of subcellular volumes improves the sensitivity of molecular organization, chemical environment, and diffusion measurements through the use of volumetric sub-cellular segmentation. We first demonstrate our approach by measuring the molecular organization of a nuclear-localized HaloTag protein relative to cell nuclei. Next, we characterize the molecular diffusion of the soluble protein, calreticulin, in the context of

Indexed as

Single Molecule ImagingDiffusionHumansdiffusionmicroscopymolecular motionsingle‐molecule tracking

Identifiers

PMID41537644
PMCPMC12895227

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.