Evidence map›Paper›PMID 41540688›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2026

[Temporal changes of chronic postsurgical pain in mice: the regulatory role of CX3CL1 in the dorsal root ganglion].

Bei Zhao, Zhengyi Lü, Dingru Ji, Shuxin Tian, Yuxin Wu, Xingzhen Li, Jie Zhou, Jianqiao Fang, Yi Liang

Abstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

9 authors.

Bei ZhaoZhejiang Provincial Key Laboratory of Acupuncture and Neurology, Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Zhengyi LüDepartment of Acupuncture and Massage, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China.
Dingru JiZhejiang Provincial Key Laboratory of Acupuncture and Neurology, Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Shuxin TianZhejiang Provincial Key Laboratory of Acupuncture and Neurology, Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Yuxin WuZhejiang Provincial Key Laboratory of Acupuncture and Neurology, Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Xingzhen LiZhejiang Provincial Key Laboratory of Acupuncture and Neurology, Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Jie ZhouZhejiang Provincial Key Laboratory of Acupuncture and Neurology, Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Jianqiao FangZhejiang Provincial Key Laboratory of Acupuncture and Neurology, Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Yi LiangZhejiang Provincial Key Laboratory of Acupuncture and Neurology, Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China.

Funding

National Natural Science Foundation of China 82174510
6 · The paper itself

Abstract

objectivesTo observe temporal changes of pain-related behaviors in mice with chronic postsurgical pain (CPSP) and identify its key mediators in the dorsal root ganglion (DRG).

methodsIn mouse models of CPSP induced by plantar incision (INC) followed by a dorsal foot injection of prostaglandin E2 (PGE2) and sham-operated mice, mechanical paw withdrawal thresholds (PWTs), thermal paw withdrawal latencies (PWLs), and cold withdrawal durations (WDs) were measured at different time points after modeling. Gene expression profiling of the DRG with RNA sequencing was performed on day 1 and day 8 after PGE2 injection. Bioinformatics analyses were conducted to explore the key mediators in the DRG for regulating CPSP, and the candidate genes and proteins were validated using RT-qPCR and ELISA. The effects of intrathecal injection of a CX3CL1-neuralizing antibody or JMS-17-2 (a CX3CR1 antagonist) on CPSP were observed.

resultsIn CPSP mouse models, incision-induced pain was resolved within 14 days, and PWTs and WDs decreased progressively till day 10 and day 12 after PGE2 injection, respectively, without significant changes in PWLs. RNA-Seq identified 975 differentially expressed genes (DEGs) on day 1 and 895 on day 8 following CPSP modeling, including 524 intersecting DEGs enriched in cell membrane, plasma membrane, and CX3C chemokine receptor binding. Cx3cl1 and Cxcl14 were the top two upregulated chemokine-related DEGs in early CPSP, whose mRNA and protein expression increased significantly in the ipsilateral DRG on day 1 but declined on day 8. Intrathecal injection of the CX3CL1-neutralizing antibody before PGE2 injection prevented CPSP development, while JMS-17-2 partially reversed CPSP during the maintenance phase.

conclusionsThis CPSP mouse model shows persistent mechanical and cold allodynia for at least 10 days after PGE2 injection without significant changes in heat hypersensitivity. CX3CL1 and related chemokine signaling in the DRG may contribute to the development of CPSP.

Indexed as

Chemokine CX3CL1Chronic PainGanglia, SpinalPostoperative PainAnimalsDisease Models, AnimalMaleMiceChemokine CX3CL1Cx3cl1 protein, mousechemokinechronic postsurgical painCX3CL1CXCL14RNA-seq

Identifiers

PMID41540688
PMCPMC12809017

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.