Evidence map›Paper›PMID 41540766›Full record

Trial reportBrain and behavior2026

Adaptogenic Effects of Mushroom Blend Supplementation on Stress, Fatigue, and Sleep: A Randomised, Double-Blind, and Placebo-Controlled Trial.

Ahmad Safiyyu'd-Din Hisamuddin, Faiqah Ramli, Teik Kee Leo, Mohamad Shazeli Che Zain, Mei Szin Wong, Mohd Raili Suhaili, Le Jie Lee, Tze Yan Lee

Abstract readRandomized Controlled Trial
In one paragraph

Trial report in Brain and behavior, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Trial
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ahmad Safiyyu'd-Din HisamuddinResearch and Development Department, Nexus Wise Sdn Bhd, Petaling Jaya, Malaysia.
Faiqah RamliResearch and Development Department, Nexus Wise Sdn Bhd, Petaling Jaya, Malaysia.ORCID https://orcid.org/0000-0003-4020-3366
Teik Kee LeoResearch and Development Department, Nexus Wise Sdn Bhd, Petaling Jaya, Malaysia.
Mohamad Shazeli Che ZainResearch and Development Department, Nexus Wise Sdn Bhd, Petaling Jaya, Malaysia.
Mei Szin WongFaculty of Medicine, Nursing and Health Sciences, SEGi University, Petaling Jaya, Selangor, Malaysia.
Mohd Raili SuhailiFaculty of Medicine, Nursing and Health Sciences, SEGi University, Petaling Jaya, Selangor, Malaysia.
Le Jie LeePrima Nexus Sdn. Bhd., Bandar Puteri Puchong, Selangor, Malaysia.
Tze Yan LeeClinical Laboratory Science Section, Institute of Medical Science Technology, Universiti Kuala Lumpur, Kajang, Selangor, Malaysia.

Funding

Nexus Wise Sdn Bhd
6 · The paper itself

Abstract

BACKGROUND/

objectivesMedicinal mushrooms have been gaining increasing attention as functional foods; however, scientific evidence from human studies remains limited.

methodsIn this study, 50 participants were randomly assigned to receive either Restake or a placebo. Psychological and physiological parameters were assessed at baseline, 6 weeks, and 12 weeks using validated tools, including the Pittsburgh Sleep Quality Index (PSQI), Visual Analog Scale for Fatigue (VAS-F), Multidimensional Fatigue Inventory (MFI), State-Trait Anxiety Inventory (STAI-S), Perceived Stress Scale (PSS), Hamilton Anxiety Scale (HAM-A), and Beck Depression Inventory (BDI). Serum biomarkers-cortisol, norepinephrine (NE), melatonin, adrenocorticotropic hormone (ACTH), and C-reactive protein (CRP)-were analyzed via ELISA.

resultsAnxiety, assessed by STAI-S and HAM-A, showed greater reductions in the Restake group at both 6 weeks (STAI-S: p = 0.025; HAM-A: p = 0.002) and 12 weeks (STAI-S: p = 0.011; HAM-A: p = 0.002). Depression (BDI) scores significantly decreased at 6 weeks (p < 0.001) and 12 weeks (p = 0.008). Fatigue levels showed significant reductions in general fatigue (p = 0.043), physical fatigue (p = 0.027), and mental fatigue (p = 0.043). Restake supplementation led to reductions in sleep quality scores (PSQI) at 6 weeks (p = 0.005) and 12 weeks (p < 0.001). Biomarker analysis revealed significant reductions (p < 0.001) in cortisol and ACTH levels and a decrease in CRP levels (p = 0.042). NE levels significantly (p = 0.033). Compared to the placebo group, Restake supplementation exhibited an increased morning melatonin trend after 12 weeks of intervention.

conclusionsRestake supplementation was well tolerated and effectively reduced psychological stress, fatigue, and improved sleep quality without adverse effects.

Indexed as

AgaricalesFatigueSleepStress, PsychologicalAdultAnxietyBiomarkersC-Reactive ProteinDepressionDietary SupplementsDouble-Blind MethodFemaleHumansHydrocortisoneMaleMelatoninBiomarkersC-Reactive ProteinHydrocortisoneMelatoninadaptogenmushroomsstressβ‐glucan

Identifiers

PMID41540766
PMCPMC12808922

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.