Evidence map›Paper›PMID 41542043›Full record

ArticleResearch square2026

Characterization of dietary choline uptake by the gut microbiome reveals choline assimilating microbes and influences on host choline metabolism.

Elizabeth Johnson, Paula Bañuelos, Janine Comrie, Annett Richter, Henry Le, Sharon Thompson

Abstract readPreprint
In one paragraph

Article in Research square, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Elizabeth JohnsonCornell University.ORCID 0000-0002-8290-1122
Paula BañuelosCornell University.
Janine ComrieCornell University.ORCID 0000-0003-3747-3277
Annett RichterCornell University.
Henry LeCornell University.ORCID 0000-0003-2942-2357
Sharon ThompsonCornell University.

Funding

Sphingolipid-dependent host-microbe interactionsR35GM138281 · NIGMS · CORNELL UNIVERSITY · PI Elizabeth L Johnson · 2020 to 2026
$3.1M
NIGMS NIH HHS R35 GM138281
6 · The paper itself

Abstract

Choline is an essential nutrient with diverse roles in host metabolism; however, the current understanding of its microbial fate is largely restricted to trimethylamine production. Here, we apply the BioOrthogonal-labeling, Sorting, Sequencing, and mass Spectrometry (BOSSS) workflow to map dietary choline-specific gut microbial interactions. Using an alkyne-modified choline analog (propargylcholine) in mice, we fluorescently label and flow-sort choline-assimilating gut bacteria, identifying a varied set of taxa enriched in probiotic species, including

Indexed as

cholineclick chemistrydiet-microbiomegut microbiomenutrition

Identifiers

PMID41542043
PMCPMC12803358

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.