Evidence map›Paper›PMID 41547681›Full record

ArticleIn vitro cellular & developmental biology. Animal2026

Effect of cAMP concentration on apoptosis of sheep granulosa cells in vitro.

Wenhao Wang, Linlin Pei, Peilin Guo, Andi Qiao, Shuyan Guo, Xin Xu, Chunjie Liu

Abstract read
PubMed Publisher
In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Wenhao WangCollege of Animal Science and Technology, Key Laboratory of Livestock and Forage Resources Utilization Around Tarim and Key Laboratory of Tarim Animal Husbandry Science and Technology, Tarim University, Alar, 843300, China.
Linlin PeiCollege of Animal Science and Technology, Key Laboratory of Livestock and Forage Resources Utilization Around Tarim and Key Laboratory of Tarim Animal Husbandry Science and Technology, Tarim University, Alar, 843300, China.
Peilin GuoCollege of Animal Science and Technology, Key Laboratory of Livestock and Forage Resources Utilization Around Tarim and Key Laboratory of Tarim Animal Husbandry Science and Technology, Tarim University, Alar, 843300, China.
Andi QiaoCollege of Animal Science and Technology, Key Laboratory of Livestock and Forage Resources Utilization Around Tarim and Key Laboratory of Tarim Animal Husbandry Science and Technology, Tarim University, Alar, 843300, China.
Shuyan GuoCollege of Animal Science and Technology, Key Laboratory of Livestock and Forage Resources Utilization Around Tarim and Key Laboratory of Tarim Animal Husbandry Science and Technology, Tarim University, Alar, 843300, China.
Xin XuCollege of Life Science and Technology, State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in Tarim Basin, Tarim University, Alar, 843300, China. 1241141697@qq.com.
Chunjie LiuCollege of Animal Science and Technology, Key Laboratory of Livestock and Forage Resources Utilization Around Tarim and Key Laboratory of Tarim Animal Husbandry Science and Technology, Tarim University, Alar, 843300, China. guilt369@163.com.

Funding

Tarim University TDGRI202238Tarim University TDZKBS202102
6 · The paper itself

Abstract

Cyclic adenosine monophosphate (cAMP), an intracellular messenger, regulates granulosa cell (GC) proliferation, differentiation, and apoptosis via downstream effectors like PKA. Previous studies focused on cAMP promoting GC proliferation and its unidirectional transfer from GC to oocytes through gap junctions to sustain meiotic arrest. This study hypothesized cAMP bidirectionally interacts between GC and oocytes: partially entering oocytes to maintain meiotic arrest, while regulating GC apoptosis in a concentration-dependent manner. Immunofluorescence confirmed intracellular cAMP signaling in GC. Treatment with 0-10 μmol/L cAMP for 48 h revealed dual effects: low concentrations (2-4 μmol/L) significantly suppressed apoptosis and enhanced viability (CCK-8 assay), whereas concentrations > 4 μmol/L increased apoptosis and inhibited proliferation. This study provides new insights into the direct regulatory role of cAMP in sheep GC, emphasizes its dual role in cell survival and apoptosis, and deepens our understanding of the mechanism of follicular development in sheep.

Indexed as

ApoptosisCyclic AMPGranulosa CellsAnimalsCell ProliferationCell SurvivalFemaleMeiosisOocytesSheepSignal TransductionCyclic AMPApoptosiscAMPGranulosa cellsSheep

Identifiers

PMID41547681

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.