Evidence map›Paper›PMID 41548688›Full record

ArticleMetabolic engineering2026

Investigating overflow metabolism in heterotrophic cultures of the green alga Chromochloris zofingiensis.

Michelle Meagher, Dimitrios J Camacho, Sean D Gallaher, Sabeeha S Merchant, Nanette R Boyle

Abstract read
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In one paragraph

Article in Metabolic engineering, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Michelle MeagherChemical and Biological Engineering Department, Colorado School of Mines, Golden, CO, 80401, USA.
Dimitrios J CamachoDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, 94720, USA.
Sean D GallaherCalifornia Institute for Quantitative Biosciences (QB3), University of California, Berkeley, CA, 94720, USA.
Sabeeha S MerchantDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, 94720, USA; California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, CA, 94720, USA; Department of Plant and Microbial Biology, University of California, Berkeley, CA, 994720, USA; Environmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Nanette R BoyleChemical and Biological Engineering Department, Colorado School of Mines, Golden, CO, 80401, USA; Quantitative Biosciences and Engineering, Colorado School of Mines, Golden, CO, 80401, USA. Electronic address: nboyle@mines.edu.

Funding

THE MOLECULAR BASIS OF CELL FUNCTIONT32GM007232 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI BILDER, DAVID, UNAL, ELCIN · 1985 to 2022
$36.4M
NIGMS NIH HHS T32 GM007232
6 · The paper itself

Abstract

Chromochloris zofingiensis is of interest for its ability to perform a reversible trophic switch in the presence of glucose that is characterized by a shutdown of photosynthesis and an accumulation of energy storage metabolites. Previous work has shown that this trophic switch is accompanied by overflow metabolism and the production of lactate in aerobic conditions. This trophic switch is not observed in nutrient replete media. We utilized isotopically assisted metabolic flux analysis to characterize intracellular flux distributions that are associated with different metabolic phenotypes observed in this organism in different media formulations in light and dark conditions. The results of this analysis showed that low iron cultures have no flux through carbon fixation reactions, and that the carbon flux entering the TCA cycle in these cultures is approximately 40 % lower than that in iron replete cultures grown heterotrophically. This analysis was complemented with transcriptomics data collected for C. zofingiensis grown in iron limited conditions to provide further evidence towards the negative impact of iron limitation on both photosynthetic and respiratory activity. Overflow metabolism allows this alga to compensate for the lower energy production that results from iron limitation. This work highlights how nutrient availability can lead to changes in the metabolism of C. zofingiensis.

Indexed as

ChlorophytaCitric Acid CycleHeterotrophic ProcessesIronPhotosynthesisMetabolic Flux AnalysisIronIron metabolismMetabolic flux analysisMetabolic modeling

Identifiers

PMID41548688
PMCPMC13564474

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.