Evidence map›Paper›PMID 41553530›Full record

ArticleArchives of microbiology2026

WGS analysis and functional studies illustrate promising gene-signatures for probiotic attributes and molecular-targeted therapeutic prospects of Lactiplantibacillus plantarum LP-ARP2.

Sinjini Patra, Ritwik Patra, Pradeep Kumar Das, Anshuman Dixit, Anasuya Roychowdhury

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Article in Archives of microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Sinjini PatraBiochemistry and Cell Biology Laboratory, School of Basic Sciences, Indian Institute of Technology Bhubaneswar, Bhubaneswar, Odisha, 752050, India.
Ritwik PatraComputational Biology and Bioinformatics Laboratory, Institute of Life Sciences, Nalco Square, Bhubaneswar, Odisha, 751023, India.
Pradeep Kumar DasComputational Biology and Bioinformatics Laboratory, Institute of Life Sciences, Nalco Square, Bhubaneswar, Odisha, 751023, India.
Anshuman DixitComputational Biology and Bioinformatics Laboratory, Institute of Life Sciences, Nalco Square, Bhubaneswar, Odisha, 751023, India.
Anasuya RoychowdhuryBiochemistry and Cell Biology Laboratory, School of Basic Sciences, Indian Institute of Technology Bhubaneswar, Bhubaneswar, Odisha, 752050, India. aroychowdhury@iitbbs.ac.in.ORCID https://orcid.org/0000-0003-3735-3021

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gene-signatures for probiotic attributes expedite screening of novel probiotic strains as well as identify molecular basis of probiotic benefits. Therefore, strain-specific genomic-studies correlating functional-assays are in demand. Here, we present the same for Lactiplantibacillus plantarum LP-ARP2 using genomic-metabolomic-functional approaches. Genomic-features of LP-ARP2 are further compared with clinically relevant L. plantarum strains to elucidate gene-specific commonality. We find robust stress-resilience genetic set-up (usp/hsl/clp/ABC-transporters/ ATP-synthase/chaperons dna/gro/grp) in LP-ARP2-genome. Our acid/bile tolerance assays also indicate survivability (> 60%) of the strain in harsh conditions. Presence of adhesion-related (lspA/mapA/eno/srtA/glycosyltransferases/glycosylhydrolases/lipoproteins) and biofilm-forming genes (veg/luxS) further align with its efficacy in autoaggregation (> 60%), adhesion (Caco-2), and biofilm-formation (24 h). CAZyme-genes with significant prebiotic utilization indicate the strain's ability for gut-microbial-modulation and adaptation. Metabolic-profiling of LP-ARP2-derived-CFS (HRMS analysis) validates the presence of related genes for SCFAs/vitamins/amino-acids/neurotransmitters GABA/serotonin/acetylcholine etc. Besides, many metabolites are reported antimicrobials. Indeed LP-ARP2 shows significant antibacterial potential against multidrug-resistant bacteria (Gram-positive/Gram-negative), gut-pathogen Salmonella Typhimurium and pathogenic-biofilm (MRSA). Presence of antioxidant-genes in LP-ARP2-genome (thioredoxin/NADH-dependent-nox/npr/ndh/glutathione-reductase/glutaredoxin/catalase/peroxidases/methionine sulfoxide reductase) are validated by high radical-scavenging activity of LP-ARP2 (ABTS > 40%, DPPH 25 U/mL, superoxide > 80%, and hydroxyl > 70%). Moreover, in-silico functional-network-analysis reveals LP-ARP2-derived metabolites target oxidative stress, neuroinflammation, amyloid-beta metabolism, tau-phosphorylation, neurogenesis, and synaptic function, indicating molecular relevance of the therapeutic potential of LP-ARP2. Fascinatingly, genomic-analysis between LP-ARP2 with clinically relevant (depression and intestinal disorders) L. plantarum strains (299v and Lp01) elucidate comparable genetic-features for beneficial probiotics. Thus, study offers potential gene-signatures for probiotic-benefits of L. plantarum and project LP-ARP2 as a promising probiotic with antibacterial, antioxidant and psychobiotic potential.

Indexed as

Lactiplantibacillus plantarumProbioticsAnti-Bacterial AgentsBacterial AdhesionBacterial ProteinsBiofilmsGenome, BacterialHumansWhole Genome SequencingAnti-Bacterial AgentsBacterial ProteinsAntioxidant and antimicrobial activitiesGene-signaturesHuman-target based functional-network analysisMetabolic profilingMicrobial dysbiosisProbioticsPsychobiotic potentialWhole genome sequencing (WGS) analysis

Identifiers

PMID41553530

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.