ArticleBMC genomics2026
Transcriptome analysis reveals KRAS as a crucial regulator of ovarian follicle development in Wuding chickens.
Article in BMC genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
backgroundThis study investigated the molecular basis of follicular development and egg production in Wuding chickens, an indigenous Chinese breed known for exceptional meat quality and nutrient-rich eggs, but suboptimal egg-laying performance, by comparing them with high-yielding Jingfen No.1 chickens.
resultsHigh-throughput transcriptome sequencing of ovarian tissues identified 6,790 differentially expressed genes (DEGs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed these DEGs were significantly implicated in reproductive pathways, including steroid biosynthesis, cell cycle, MAPK signaling, calcium signaling, oocyte meiosis, and progesterone-mediated oocyte maturation. A protein–protein interaction (PPI) network highlighted Kirsten rat sarcoma viral oncogene (KRAS) as a crucial hub gene. Further functional experiments focused on the role of KRAS in chicken ovarian granulosa cells (GCs). Overexpression of KRAS significantly enhanced the production of progesterone (P4) and estradiol (E2) and upregulated the expression of steroidogenic genes (e.g., STAR, CYP19A1, HSD3B1, PTGS2). KRAS also promoted GC proliferation by increasing cell viability, stimulating DNA synthesis, and upregulating proliferation markers (AKT1, mTOR, JAK2, STAT3, PCNA). Additionally, KRAS facilitated cell cycle progression by reducing the G0/G1 phase population and increasing the S phase, alongside upregulating cell cycle regulators (CCND1, CCND2, CCNE2, CDK2). Importantly, KRAS inhibited GC apoptosis by increasing antiapoptotic genes (BCL2, TGF-β) and decreasing proapoptotic genes (Caspase 3, BAX). Finally, KRAS delayed cellular senescence by reducing SA-β-gal activity and reciprocally regulating P21 (downregulation) and SIRT1 (upregulation).
conclusionsCollectively, these findings demonstrate that KRAS is a pivotal regulator of follicular development in Wuding chickens, influencing GC proliferation, apoptosis, steroid hormone synthesis, and senescence. This research provides valuable insights for genetic improvement programs aimed at enhancing egg production in indigenous chicken breeds.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.